SEED Subsystem: Coenzyme B12 biosynthesis in Rhodospirillum rubrum S1H

Experiments:

Role Gene Name carbon source anaerobic Succinic Acid 31.25 mM  
5,6-dimethylbenzimidazole synthase, flavin destructase family No genes
ATPase component CbiO of energizing module of cobalt ECF transporter No genes
Additional lipoprotein component of predicted cobalamin ECF transporter No genes
Additional substrate-specific component CbiN of cobalt ECF transporter No genes
Adenosylcobinamide amidohydrolase (EC 3.5.1.90) Rru_A0492 +0.4
Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62) No genes
Adenosylcobinamide-phosphate synthase No genes
Aerobic cobaltochelatase CobS subunit (EC 6.6.1.2) Rru_A0206 -2.1
Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2) Rru_A0207 -2.0
Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73) Rru_A0669 N.D.
CblX, a non-orthologous displasment for Alpha-ribazole-5'-phosphate phosphatase No genes
CblY, a non-orthologous displasment for Alpha-ribazole-5'-phosphate phosphatase No genes
CblZ, a non-orthologous displasment for Alpha-ribazole-5'-phosphate phosphatase No genes
ChlD component of cobalt chelatase involved in B12 biosynthesis No genes
ChlI component of cobalt chelatase involved in B12 biosynthesis No genes
Cob(I)alamin adenosyltransferase (EC 2.5.1.17) Rru_A3367 +0.6
Cob(I)alamin adenosyltransferase PduO (EC 2.5.1.17) No genes
Cob(II)alamin reductase No genes
CobN component of cobalt chelatase involved in B12 biosynthesis Rru_A3366 -0.5
CobW GTPase involved in cobalt insertion for B12 biosynthesis Rru_A0671 -0.7
Cobalamin biosynthesis protein CbiG No genes
Cobalamin biosynthesis protein CobG Rru_A3383 -1.1
Cobalamin synthase No genes
Cobalamine-related hypothetical metal-binding protein CrdX Rru_A3547 +0.7
Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130) Rru_A2990 -1.7
Cobalt-precorrin-3b C17-methyltransferase Rru_A2989 -2.6
Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) Rru_A3384 -2.2
Cobalt-precorrin-6 synthase, anaerobic Rru_A2996 -0.1
Cobalt-precorrin-6x reductase (EC 1.3.1.54) Rru_A2988 -2.9
Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-) Rru_A2992 -2.9
Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) Rru_A2992 -2.9
Cobalt-precorrin-8x methylmutase (EC 5.4.1.2) Rru_A2991 N.D.
Cobyric acid synthase No genes
Cobyrinic acid A,C-diamide synthase Rru_A3359 -3.0
Duplicated ATPase component CbrU of energizing module of predicted cobalamin ECF transporter No genes
HoxN/HupN/NixA family cobalt transporter No genes
HupE-UreJ family cobalt transporter No genes
L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81) Rru_A2651 -3.6
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21) Rru_A0667 -3.7
Optional hypothetical component of the B12 transporter BtuM No genes
Optional hypothetical component of the B12 transporter BtuN No genes
Outer membrane vitamin B12 receptor BtuB No genes
Precorrin-6A synthase (EC 2.1.1.152) Rru_A2970 -1.3
Predicted adenosylcobinamide-phosphate guanylyltransferase CobY (EC 2.7.7.62) No genes
Predicted alpha-ribazole-5'-phosphate phosphatase CobZ (EC 3.1.3.73) No genes
Predicted alpha-ribazole-5-phosphate synthase CblS for cobalamin biosynthesis No genes
Predicted cobalt ABC transporter periplasmic component No genes
Predicted cobalt transporter CbtA No genes
Predicted cobalt transporter CbtC Rru_A0458 -0.1
Predicted cobalt transporter in Bacteroides_Porphyromonas No genes
Predicted cobalt transporter in Mycobacteria No genes
Predicted cobalt transporter in Thermoplasma No genes
Predicted cobalt transporter in sulfate-reducing delta-proteobacteria No genes
Putative 2Fe-2S ferredoxin CbiW involved in B12 biosynthesis No genes
Sirohydrochlorin cobaltochelatase (EC 4.99.1.3) No genes
Sirohydrochlorin cobaltochelatase CbiK (EC 4.99.1.3) No genes
Substrate-specific component CbiM of cobalt ECF transporter No genes
Substrate-specific component CblT of predicted B12-regulated ECF transporter for dimethylbenzimidazole No genes
Substrate-specific component CbrT of predicted cobalamin ECF transporter No genes
Threonine kinase in B12 biosynthesis No genes
Transmembrane component CbiQ of energizing module of cobalt ECF transporter No genes
Transmembrane component CbrV of energizing module of predicted cobalamin ECF transporter No genes
Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) Rru_A1933 +0.5
  Rru_A3362 -2.4
Vitamin B12 ABC transporter, ATPase component BtuD Rru_A0491 -0.2
Vitamin B12 ABC transporter, B12-binding component BtuF Rru_A0489 -0.8
Vitamin B12 ABC transporter, permease component BtuC Rru_A0490 -3.6
  Rru_A3222 +0.5

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