SEED Subsystem: Acetyl-CoA fermentation to Butyrate in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name L-Isoleucine (C)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) Pf6N2E2_1833 +0.3
  Pf6N2E2_1850 +0.1
  Pf6N2E2_1922 -0.0
  Pf6N2E2_2290 N.D.
  Pf6N2E2_3894 -0.1
  Pf6N2E2_6000 +0.1
3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) No genes
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) No genes
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) Pf6N2E2_1722 +0.1
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) Pf6N2E2_1850 +0.1
  Pf6N2E2_1922 -0.0
  Pf6N2E2_2290 N.D.
  Pf6N2E2_3894 -0.1
  Pf6N2E2_6000 +0.1
Acetoacetate metabolism regulatory protein AtoC No genes
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) Pf6N2E2_2113 -0.2
Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8) No genes
Acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (EC 2.8.3.8) No genes
Butyrate kinase (EC 2.7.2.7) No genes
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
Butyryl-CoA dehydrogenase (EC 1.3.99.2) Pf6N2E2_1022 +0.1
  Pf6N2E2_1146 -2.1
  Pf6N2E2_1148 +0.4
  Pf6N2E2_1855 -0.1
  Pf6N2E2_5333 -0.1
  Pf6N2E2_5924 +0.1
Electron transfer flavoprotein, alpha subunit Pf6N2E2_4705 -0.1
  Pf6N2E2_5984 N.D.
Electron transfer flavoprotein, beta subunit Pf6N2E2_4706 +0.3
  Pf6N2E2_5983 N.D.
  Pf6N2E2_751 -0.5
Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) Pf6N2E2_5982 N.D.
Enoyl-CoA hydratase (EC 4.2.1.17) Pf6N2E2_1013 -0.2
  Pf6N2E2_1046 -0.1
  Pf6N2E2_1834 +0.5
  Pf6N2E2_1835 -0.3
  Pf6N2E2_1840 +0.4
  Pf6N2E2_1922 -0.0
  Pf6N2E2_1934 -0.4
  Pf6N2E2_1935 -0.2
  Pf6N2E2_2290 N.D.
  Pf6N2E2_2773 +0.2
  Pf6N2E2_3894 -0.1
  Pf6N2E2_5493 -0.1
Ferredoxin-like protein No genes
Ferredoxin-like protein FixX No genes
Ferredoxin-like protein YdiT No genes
Ferredoxin-like protein YgcO No genes
Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-) No genes
phosphotransbutyrylase (EC 2.3.1.19) No genes

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