SEED Subsystem: Serine-glyoxylate cycle in Synechococcus elongatus PCC 7942

Experiments:

Role Gene Name Flask growth in 60 uE Light-Dark 12:12  
⨯
3-ketoacyl-CoA thiolase (EC 2.3.1.16) No genes
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) Synpcc7942_1309 metF N.D.
5-FCL-like protein, but predicted not to be 5-formyltetrahydrofolate cyclo-ligase (5-FCL) No genes
5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) Synpcc7942_1300 +1.3
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) No genes
Aconitate hydratase (EC 4.2.1.3) No genes
Citrate synthase (EC 2.3.3.1) No genes
Citrate synthase (si) (EC 2.3.3.1) Synpcc7942_0612 gltA N.D.
Enolase (EC 4.2.1.11) Synpcc7942_0639 eno N.D.
Formate--tetrahydrofolate ligase (EC 6.3.4.3) No genes
Formiminotetrahydrofolate cyclodeaminase (EC 4.3.1.4) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) No genes
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) No genes
GDSL-like lipase/acylhydrolase No genes
Glycerate kinase (EC 2.7.1.31) No genes
Isocitrate lyase (EC 4.1.3.1) No genes
Malate dehydrogenase (EC 1.1.1.37) No genes
Malate synthase-related protein No genes
Malyl-CoA lyase (EC 4.1.3.24) No genes
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) Synpcc7942_0777 folD N.D.
Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) No genes
Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) No genes
Methylene tetrahydromethanopterin dehydrogenase (EC 1.5.99.9) No genes
Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) Synpcc7942_0777 folD N.D.
Methylmalonyl-CoA mutase (EC 5.4.99.2) No genes
N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27) No genes
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) No genes
Phosphoglyceromutase No genes
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) No genes
Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Serine hydroxymethyltransferase (EC 2.1.2.1) Synpcc7942_0282 glyA N.D.
Serine--glyoxylate aminotransferase (EC 2.6.1.45) Synpcc7942_0191 N.D.
Serine--pyruvate aminotransferase (EC 2.6.1.51) Synpcc7942_2160 nhtA +0.2
Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) Synpcc7942_0641 sdhA +2.2
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) Synpcc7942_1533 sdhB +2.2
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) No genes
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) No genes
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) No genes
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5) No genes
crotonyl-CoA reductase No genes
cytosolic long-chain acyl-CoA thioester hydrolase family protein No genes
decarboxylase No genes
isobutyryl-CoA dehydrogenase No genes
low-specificity D-threonine aldolase No genes
polyhydroxyalkanoate synthesis repressor PhaR No genes
putative glycerate kinase No genes
putative isobutyryl-CoA mutase, chain B No genes

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