SEED Subsystem: Proline, 4-hydroxyproline uptake and utilization in Burkholderia phytofirmans PsJN

Experiments:

Role Gene Name Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days  
1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22) BPHYT_RS22635 +0.4
4-hydroxyproline epimerase (EC 5.1.1.8) BPHYT_RS22640 +0.1
Aldehyde dehydrogenase (EC 1.2.1.3) in 4-hydroxyproline catabolic gene cluster No genes
Amino acid permease in 4-hydroxyproline catabolic gene cluster No genes
D-amino acid dehydrogenase (EC 1.4.99.1) family protein in hydroxy-L-proline catabolic cluster No genes
D-amino-acid oxidase (EC 1.4.3.3) BPHYT_RS22645 +0.0
D-proline reductase, 23 kDa subunit (EC 1.21.4.1) No genes
D-proline reductase, 26 kDa subunit (EC 1.21.4.1) No genes
D-proline reductase, 45 kDa subunit (EC 1.21.4.1) No genes
Delta 1-pyrroline-5-carboxylate dehydrogenase domain protein No genes
Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) BPHYT_RS06150 +0.6
  BPHYT_RS19355 +1.3
Dipeptidyl peptidase IV in 4-hydroxyproline catabolic gene cluster No genes
Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain No genes
Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) BPHYT_RS24140 +0.2
  BPHYT_RS28455 +0.0
L-Proline/Glycine betaine transporter ProP BPHYT_RS10805 +0.6
  BPHYT_RS12995 N.D.
  BPHYT_RS20435 +0.2
  BPHYT_RS22625 +0.2
  BPHYT_RS25640 -0.2
  BPHYT_RS28490 -0.4
  BPHYT_RS28670 +0.4
  BPHYT_RS28710 +0.6
  BPHYT_RS28755 N.D.
  BPHYT_RS28835 -0.6
  BPHYT_RS31705 -0.2
Microbial collagenase, secreted (EC 3.4.24.3) No genes
Not a Proline racemase, nor 4-hydroxyproline epimerase [missing catalytic residues] BPHYT_RS28265 -0.1
Predicted 4-hydroxyproline dipeptidase No genes
Predicted regulator PutR for proline utilization, GntR family BPHYT_RS11040 -0.8
  BPHYT_RS15065 +0.4
  BPHYT_RS23045 N.D.
  BPHYT_RS23770 -0.9
  BPHYT_RS23940 +0.6
  BPHYT_RS30810 -0.4
  BPHYT_RS34445 +0.1
ProQ: influences osmotic activation of compatible solute ProP No genes
Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) BPHYT_RS19355 +1.3
Proline iminopeptidase (EC 3.4.11.5) No genes
Proline racemase (EC 5.1.1.4) No genes
Proline-specific permease proY No genes
Proline/sodium symporter PutP (TC 2.A.21.2.1) No genes
PutR, transcriptional activator of PutA and PutP No genes
Putative beta-galactosidase in 4-hydroxyproline catabolic gene cluster No genes
Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster BPHYT_RS22650 +0.3
Similar to eukaryotic Peptidyl prolyl 4-hydroxylase, alpha subunit (EC 1.14.11.2) BPHYT_RS26875 -0.1
Transcriptional repressor of PutA and PutP BPHYT_RS19355 +1.3
Ureidoglycolate/malate/sulfolactate dehydrogenase family (EC 1.1.1.-) No genes
putPA fragment of control region No genes

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