SEED Subsystem: Isoleucine degradation in Pseudomonas fluorescens FW300-N2E3

Experiments:

Role Gene Name acetate (C)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) AO353_00510 +0.3
  AO353_01155 +0.2
3-ketoacyl-CoA thiolase (EC 2.3.1.16) AO353_05785 +0.0
  AO353_25685 +0.4
3-ketoacyl-CoA thiolase [isoleucine degradation] (EC 2.3.1.16) No genes
Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2) No genes
Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12) No genes
Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) AO353_15575 -0.0
  AO353_26635 -0.9
Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4) AO353_15580 +0.6
  AO353_26640 -1.6
Branched-chain amino acid aminotransferase (EC 2.6.1.42) AO353_26655 -0.9
Butyrate kinase (EC 2.7.2.7) No genes
Butyryl-CoA dehydrogenase (EC 1.3.99.2) AO353_00670 -0.2
  AO353_01500 -0.1
  AO353_15135 -0.6
  AO353_15225 +0.3
  AO353_24835 -0.2
  AO353_25670 -0.1
  AO353_25680 -0.4
  AO353_28600 +0.2
Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168) AO353_15585 +0.5
  AO353_26645 -0.6
Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4) AO353_26650 +0.1
Enoyl-CoA hydratase (EC 4.2.1.17) AO353_00510 +0.3
  AO353_03780 -0.2
  AO353_17555 -0.1
  AO353_19125 N.D.
  AO353_23055 +0.5
  AO353_24830 -1.6
Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) AO353_01155 +0.2
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.-) No genes
Predicted transcriptional regulator of leucine degradation pathway, MerR family No genes
Probable acyl-CoA dehydrogenase (EC 1.3.99.3) AO353_25690 N.D.
Transcriptional regulator BkdR of isoleucine and valine catabolism operon AO353_26630 -0.3
phosphotransbutyrylase (EC 2.3.1.19) No genes

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