SEED Subsystem: Polyhydroxybutyrate metabolism in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name Carnitine Hydrochloride (C)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) Pf6N2E2_1833 +0.2
  Pf6N2E2_1850 +0.0
  Pf6N2E2_1922 +0.1
  Pf6N2E2_2290 N.D.
  Pf6N2E2_3894 +0.1
  Pf6N2E2_6000 -0.1
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) No genes
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) Pf6N2E2_1722 +0.2
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) Pf6N2E2_1850 +0.0
  Pf6N2E2_1922 +0.1
  Pf6N2E2_2290 N.D.
  Pf6N2E2_3894 +0.1
  Pf6N2E2_6000 -0.1
3-ketoacyl-CoA thiolase (EC 2.3.1.16) Pf6N2E2_1145 N.D.
  Pf6N2E2_2539 +0.0
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetoacetyl-CoA synthetase (EC 6.2.1.16) Pf6N2E2_2042 -1.9
  Pf6N2E2_2190 +0.1
  Pf6N2E2_5827 -0.0
Acetyl-CoA acetyltransferase (EC 2.3.1.9) Pf6N2E2_2113 -1.2
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
D(-)-3-hydroxybutyrate oligomer hydrolase (EC 3.1.1.22) No genes
D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) Pf6N2E2_2041 -1.4
D-beta-hydroxybutyrate permease Pf6N2E2_2040 -1.6
Enoyl-CoA hydratase (EC 4.2.1.17) Pf6N2E2_1013 -0.1
  Pf6N2E2_1046 -0.3
  Pf6N2E2_1834 +0.6
  Pf6N2E2_1835 +0.2
  Pf6N2E2_1840 +0.2
  Pf6N2E2_1922 +0.1
  Pf6N2E2_1934 -0.2
  Pf6N2E2_1935 +0.3
  Pf6N2E2_2290 N.D.
  Pf6N2E2_2773 +0.2
  Pf6N2E2_3894 +0.1
  Pf6N2E2_5493 +0.2
Intracellular PHB depolymerase (EC 3.1.1.-) No genes
Poly(3-hydroxyalkanoate) depolymerase Pf6N2E2_3773 -0.2
Polyhydroxyalkanoate granule-associated protein PhaF Pf6N2E2_3776 N.D.
Polyhydroxyalkanoic acid synthase Pf6N2E2_3772 -0.1
  Pf6N2E2_3774 -0.0
Putative 3-hydroxyacyl-coa dehydrogenase No genes
Short chain fatty acids transporter Pf6N2E2_5193 +0.0
polyhydroxyalkanoate granule-associated protein PhaI Pf6N2E2_3777 -0.1
polyhydroxyalkanoate synthesis repressor PhaR No genes

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