SEED Subsystem: Acetyl-CoA fermentation to Butyrate in Burkholderia phytofirmans PsJN

Experiments:

Role Gene Name Parafilmed volatile agar plate with Trichoderma atroviridae IMI  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) BPHYT_RS03225 +0.1
  BPHYT_RS06760 -0.2
  BPHYT_RS09560 -0.6
  BPHYT_RS13545 -0.3
  BPHYT_RS34155 +0.3
3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) BPHYT_RS06890 -0.2
  BPHYT_RS13690 -0.0
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) BPHYT_RS22375 +0.5
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) BPHYT_RS22380 +0.5
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) BPHYT_RS09560 -0.6
  BPHYT_RS13545 -0.3
Acetoacetate metabolism regulatory protein AtoC No genes
Acetoacetyl-CoA reductase (EC 1.1.1.36) BPHYT_RS00860 -0.6
  BPHYT_RS09155 +1.4
  BPHYT_RS27640 +0.4
  BPHYT_RS29090 -0.9
Acetyl-CoA acetyltransferase (EC 2.3.1.9) No genes
Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8) BPHYT_RS07875 +0.1
  BPHYT_RS20785 +0.5
Acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (EC 2.8.3.8) No genes
Butyrate kinase (EC 2.7.2.7) No genes
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
Butyryl-CoA dehydrogenase (EC 1.3.99.2) BPHYT_RS00670 +0.3
  BPHYT_RS02220 -0.3
  BPHYT_RS08290 +0.1
  BPHYT_RS20325 +0.2
  BPHYT_RS25370 +0.2
  BPHYT_RS25400 +2.8
  BPHYT_RS28040 -0.3
Electron transfer flavoprotein, alpha subunit BPHYT_RS14800 N.D.
  BPHYT_RS20830 +0.0
  BPHYT_RS25205 +0.3
Electron transfer flavoprotein, beta subunit BPHYT_RS14805 N.D.
  BPHYT_RS20825 N.D.
  BPHYT_RS25210 +0.5
  BPHYT_RS31455 N.D.
Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) BPHYT_RS13695 N.D.
Enoyl-CoA hydratase (EC 4.2.1.17) BPHYT_RS02345 +0.6
  BPHYT_RS03225 +0.1
  BPHYT_RS03235 -0.1
  BPHYT_RS03825 N.D.
  BPHYT_RS08375 N.D.
  BPHYT_RS08630 -0.2
  BPHYT_RS09560 -0.6
  BPHYT_RS10815 N.D.
  BPHYT_RS11780 N.D.
  BPHYT_RS13545 -0.3
  BPHYT_RS14830 -0.2
  BPHYT_RS25360 N.D.
  BPHYT_RS25695 N.D.
  BPHYT_RS27105 +0.1
  BPHYT_RS27895 -0.2
  BPHYT_RS28620 +0.6
  BPHYT_RS35465 -0.4
Ferredoxin-like protein No genes
Ferredoxin-like protein FixX No genes
Ferredoxin-like protein YdiT No genes
Ferredoxin-like protein YgcO No genes
Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-) No genes
phosphotransbutyrylase (EC 2.3.1.19) No genes

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