SEED Subsystem: Heme and Siroheme Biosynthesis in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C  
5-aminolevulinate synthase (EC 2.3.1.37) No genes
Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3) Pf6N2E2_4129 N.D.
Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22) Pf6N2E2_1410 +0.2
  Pf6N2E2_1411 -1.4
  Pf6N2E2_2321 +0.3
Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), divergent, putative No genes
Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), divergent, putative 2 No genes
Distant homolog of E. coli HemX protein in Xanthomonadaceae No genes
Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1) Pf6N2E2_5233 N.D.
Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8) Pf6N2E2_1739 -0.1
  Pf6N2E2_5022 N.D.
Glutamyl-tRNA reductase (EC 1.2.1.70) Pf6N2E2_5220 N.D.
Glutamyl-tRNA synthetase (EC 6.1.1.17) No genes
HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductase No genes
Hemoprotein HemQ, essential component of heme biosynthetic pathway in Gram-positive bacteria No genes
Hypothetical membrane protein, possible involvement in cytochrome functioning/assembly No genes
Hypothetical radical SAM family enzyme, NOT coproporphyrinogen III oxidase, oxygen-independent No genes
Porphobilinogen deaminase (EC 2.5.1.61) Pf6N2E2_4431 N.D.
Porphobilinogen synthase (EC 4.2.1.24) Pf6N2E2_2146 +0.2
  Pf6N2E2_4452 +0.2
Precorrin-2 oxidase (EC 1.3.1.76) Pf6N2E2_302 -0.2
Protoporphyrinogen IX oxidase, aerobic (EC 1.3.3.4) No genes
Protoporphyrinogen IX oxidase, oxygen-independent, HemG (EC 1.3.-.-) No genes
Putative coproporphyrinogen III oxidase of BS HemN-type, oxygen-independent (EC 1.3.99.22), in heat shock gene cluster No genes
Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB Pf6N2E2_4593 +0.3
Radical SAM family protein HutW, similar to coproporphyrinogen III oxidase, oxygen-independent, associated with heme uptake No genes
Sirohydrochlorin ferrochelatase (EC 4.99.1.4) Pf6N2E2_302 -0.2
Uroporphyrinogen III decarboxylase (EC 4.1.1.37) Pf6N2E2_3750 N.D.
Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) Pf6N2E2_2381 -0.4
  Pf6N2E2_302 -0.2
  Pf6N2E2_696 -0.5
Uroporphyrinogen-III synthase (EC 4.2.1.75) Pf6N2E2_4432 -0.1
Uroporphyrinogen-III synthase, divergent, Flavobacterial type (EC 4.2.1.75) No genes
Uroporphyrinogen-III synthase, divergent, Francisella type (EC 4.2.1.75) No genes

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