SEED Subsystem: Entner-Doudoroff Pathway in Pseudomonas fluorescens SBW25-INTG

Experiments:

Role Gene Name D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride  
⨯
2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1) PFLU_RS01655 N.D.
2,3-bisphosphoglycerate-independent phosphoglycerate mutase, archaeal type (EC 5.4.2.1) No genes
2-dehydro-3-deoxygluconate aldolase (EC 4.1.2-) No genes
2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45) PFLU_RS12820 +0.2
2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14) No genes
6-phosphogluconolactonase (EC 3.1.1.31) No genes
6-phosphogluconolactonase (EC 3.1.1.31), eukaryotic type PFLU_RS23720 N.D.
Aldehyde dehydrogenase (EC 1.2.1.3) PFLU_RS07400 -0.3
  PFLU_RS09895 -0.6
  PFLU_RS11535 -0.1
  PFLU_RS15055 -0.2
  PFLU_RS15075 -0.0
  PFLU_RS15110 -0.3
  PFLU_RS19825 -0.1
  PFLU_RS26745 -1.9
  PFLU_RS29420 -1.9
D-glycerate 2-kinase (EC 2.7.1.-) PFLU_RS08855 +0.4
Enolase (EC 4.2.1.11) PFLU_RS06375 N.D.
  PFLU_RS09395 -0.3
Glucokinase (EC 2.7.1.2) PFLU_RS24325 -0.0
Gluconate dehydratase (EC 4.2.1.39) No genes
Gluconokinase (EC 2.7.1.12) PFLU_RS23590 -0.8
Gluconolactonase (EC 3.1.1.17) PFLU_RS16870 -0.1
Glucose 1-dehydrogenase (EC 1.1.1.47) PFLU_RS10290 -0.1
  PFLU_RS14630 -0.1
Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2) PFLU_RS05385 +0.1
Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) PFLU_RS13140 +0.2
  PFLU_RS23725 -0.6
Hexokinase (EC 2.7.1.1) No genes
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) PFLU_RS24335 +0.4
Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (NAD) No genes
Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (NADP) (EC 1.2.1.9) No genes
OpcA, an allosteric effector of glucose-6-phosphate dehydrogenase, actinobacterial No genes
OpcA, an allosteric effector of glucose-6-phosphate dehydrogenase, cyanobacterial No genes
Phosphogluconate dehydratase (EC 4.2.1.12) PFLU_RS24330 -0.3
Phosphoglycerate kinase (EC 2.7.2.3) PFLU_RS28085 N.D.
Phosphoglycerate mutase (EC 5.4.2.1) No genes
Polyphosphate glucokinase (EC 2.7.1.63) No genes
Pyruvate kinase (EC 2.7.1.40) PFLU_RS08850 +0.5
  PFLU_RS24255 -0.9

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