SEED Subsystem: TCA Cycle in Klebsiella michiganensis M5al

Experiments:

Role Gene Name rhizosphere EcoFAB sample 3; outgrowth in LB  
⨯
2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) BWI76_RS08415 N.D.
Aconitate hydratase (EC 4.2.1.3) BWI76_RS11940 +0.1
Aconitate hydratase 2 (EC 4.2.1.3) BWI76_RS04910 N.D.
Aconitate hydratase X, predicted No genes
Aconitate hydratase large subunit (EC 4.2.1.3) No genes
Aconitate hydratase small subunit (EC 4.2.1.3) No genes
Archaeal succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) No genes
Archaeal succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) No genes
Citrate lyase gamma chain, acyl carrier protein (EC 4.1.3.6) No genes
Citrate synthase (si) (EC 2.3.3.1) BWI76_RS08385 -6.1
  BWI76_RS19140 +0.4
Dihydrolipoamide dehydrogenase (EC 1.8.1.4) No genes
Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4) No genes
Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4) BWI76_RS14145 +0.9
Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) BWI76_RS08420 N.D.
Fumarate hydratase class I (EC 4.2.1.2) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) BWI76_RS08450 +0.0
  BWI76_RS16115 -0.4
  BWI76_RS16275 -1.4
Fumarate hydratase class II (EC 4.2.1.2) BWI76_RS16270 -1.3
Isocitrate dehydrogenase [NADP] (EC 1.1.1.42) BWI76_RS11330 N.D.
Isocitrate dehydrogenase [NAD] (EC 1.1.1.41) No genes
Malate dehydrogenase (EC 1.1.1.37) BWI76_RS16775 +0.1
  BWI76_RS25420 -5.8
Malate:quinone oxidoreductase (EC 1.1.5.4) BWI76_RS16065 -1.1
  BWI76_RS19870 -0.1
Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) BWI76_RS02455 -1.2
  BWI76_RS08405 -5.4
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) BWI76_RS02450 -1.1
  BWI76_RS08410 N.D.
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) BWI76_RS08430 -5.3
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) BWI76_RS08425 -4.0
Transcriptional regulator of succinyl CoA synthetase operon No genes
hypothetical protein that often co-occurs with aconitase No genes

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