SEED Subsystem: Branched-Chain Amino Acid Biosynthesis in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine  
⨯
(R)-citramalate synthase (EC 2.3.1.182) No genes
2-isopropylmalate synthase (EC 2.3.3.13) Pf6N2E2_3170 -1.7
3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) Pf6N2E2_63 -2.3
3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) Pf6N2E2_64 -4.1
3-isopropylmalate dehydrogenase (EC 1.1.1.85) Pf6N2E2_66 -2.5
Acetolactate synthase large subunit (EC 2.2.1.6) Pf6N2E2_5175 -1.1
Acetolactate synthase small subunit (EC 2.2.1.6) Pf6N2E2_5176 -1.3
Acetolactate synthase small subunit (EC 2.2.1.6), Xanthomonadales type No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted, Archaeal type No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted, Archaeal type 2 No genes
Branched-chain amino acid aminotransferase (EC 2.6.1.42) Pf6N2E2_477 -1.3
Dihydroxy-acid dehydratase (EC 4.2.1.9) Pf6N2E2_4564 -2.5
Ketol-acid reductoisomerase (EC 1.1.1.86) Pf6N2E2_5177 -3.2
Leucine dehydrogenase (EC 1.4.1.9) Pf6N2E2_5294 +0.3
Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system Pf6N2E2_1065 -0.2
  Pf6N2E2_1821 -0.3
  Pf6N2E2_375 -0.0
  Pf6N2E2_4390 -1.6
  Pf6N2E2_4962 +0.2
  Pf6N2E2_5044 +0.4
  Pf6N2E2_5899 -2.9
Threonine dehydratase (EC 4.3.1.19) Pf6N2E2_1562 -0.0
Threonine dehydratase biosynthetic (EC 4.3.1.19) Pf6N2E2_3938 -0.9
  Pf6N2E2_802 +0.1
Threonine dehydratase, catabolic (EC 4.3.1.19) No genes
Valine--pyruvate aminotransferase (EC 2.6.1.66) No genes

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