SEED Subsystem: Methionine Biosynthesis in Klebsiella michiganensis M5al

Experiments:

Role Gene Name Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL  
⨯
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) BWI76_RS00805 -3.4
5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) BWI76_RS01685 +0.3
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) BWI76_RS01280 -2.0
  BWI76_RS15390 -0.4
Adenosylhomocysteinase (EC 3.3.1.1) BWI76_RS18745 -0.0
Archaeal S-adenosylmethionine synthetase (EC 2.5.1.6) No genes
Betaine--homocysteine S-methyltransferase (EC 2.1.1.5) No genes
Cystathionine beta-lyase (EC 4.4.1.8) BWI76_RS24285 -4.2
Cystathionine beta-lyase, type II (EC 4.4.1.8) No genes
Cystathionine beta-synthase (EC 4.2.1.22) BWI76_RS24205 +0.1
Cystathionine gamma-lyase (EC 4.4.1.1) BWI76_RS24210 -0.0
Cystathionine gamma-synthase (EC 2.5.1.48) BWI76_RS00785 -4.0
  BWI76_RS16730 -0.2
Cysteine synthase (EC 2.5.1.47) BWI76_RS20640 -1.1
Duplicated ATPase component MtsB of energizing module of methionine-regulated ECF transporter No genes
Homocysteine S-methyltransferase (EC 2.1.1.10) BWI76_RS06030 -0.6
Homoserine O-acetyltransferase (EC 2.3.1.31) No genes
Homoserine O-succinyltransferase (EC 2.3.1.46) BWI76_RS01655 -4.4
Homoserine dehydrogenase (EC 1.1.1.3) BWI76_RS00790 -0.0
  BWI76_RS04245 -5.5
Homoserine kinase (EC 2.7.1.39) BWI76_RS04250 -5.5
Methionine ABC transporter ATP-binding protein BWI76_RS05365 -0.3
  BWI76_RS15030 +0.4
  BWI76_RS16675 +0.8
Methionine ABC transporter permease protein BWI76_RS05360 -0.3
  BWI76_RS15035 +0.5
  BWI76_RS16665 -0.0
Methionine ABC transporter substrate-binding protein BWI76_RS05355 +0.2
  BWI76_RS15025 N.D.
  BWI76_RS16320 +0.4
  BWI76_RS16670 +0.6
  BWI76_RS17105 +0.2
  BWI76_RS27655 -0.2
Methionine repressor MetJ BWI76_RS00780 N.D.
Methionine synthase activation domain (EC 2.1.1.13) No genes
Methionine transporter MetT No genes
O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) No genes
O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) No genes
Predicted regulator of methionine metabolism, ArsR family No genes
S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) BWI76_RS05170 N.D.
S-adenosylmethionine synthetase (EC 2.5.1.6) BWI76_RS24050 N.D.
S-ribosylhomocysteine lyase (EC 4.4.1.21) BWI76_RS22115 +0.2
SAM-dependent methlytransferase UbiG No genes
SAM-dependent methlytransferase YrrT No genes
Serine acetyltransferase (EC 2.3.1.30) BWI76_RS05560 N.D.
  BWI76_RS27205 N.D.
Substrate-specific component MtsA of methionine-regulated ECF transporter No genes
Transcriptional activator MetR BWI76_RS01275 -3.4
Transmembrane component MtsC of energizing module of methionine-regulated ECF transporter No genes

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