SEED Subsystem: Chorismate Synthesis in Klebsiella michiganensis M5al

Experiments:

Role Gene Name RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x  
⨯
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase (EC 2.5.1.-) BWI76_RS14880 -0.0
2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I alpha (EC 2.5.1.54) BWI76_RS08600 -0.2
  BWI76_RS17075 +0.5
  BWI76_RS21480 -0.5
2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54) No genes
2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase II (EC 2.5.1.54) BWI76_RS15085 +0.3
3,7-dideoxy-D-threo-hepto-2, 6-diulosonate synthase (EC 4.2.3.-) No genes
3-dehydroquinate dehydratase I (EC 4.2.1.10) BWI76_RS04645 -3.2
3-dehydroquinate dehydratase II (EC 4.2.1.10) No genes
3-dehydroquinate synthase (EC 4.2.3.4) BWI76_RS26085 +0.1
4-amino, 4-deoxychorismate mutase (EC 5.4.99.-) No genes
4-amino, 4-deoxyprephenate dehydrogenase (EC 1.3.1.-) No genes
5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) BWI76_RS09760 -3.9
Arogenate dehydrogenase (EC 1.3.1.43) No genes
Chorismate mutase I (EC 5.4.99.5) BWI76_RS21465 -3.1
  BWI76_RS21475 -2.2
Chorismate mutase II (EC 5.4.99.5) No genes
Chorismate synthase (EC 4.2.3.5) BWI76_RS20435 -0.8
Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) BWI76_RS21475 -2.2
Periplasmic chorismate mutase I precursor (EC 5.4.99.5) No genes
Prephenate and/or arogenate dehydrogenase (unknown specificity) (EC 1.3.1.12)(EC 1.3.1.43) No genes
Prephenate dehydratase (EC 4.2.1.51) BWI76_RS21465 -3.1
Prephenate dehydrogenase (EC 1.3.1.12) No genes
Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25) No genes
Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25) BWI76_RS13270 +0.6
  BWI76_RS16705 -0.3
Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) BWI76_RS06460 -0.1
  BWI76_RS16935 +0.9
  BWI76_RS25645 -2.4
Shikimate 5-dehydrogenase I gamma (EC 1.1.1.25) BWI76_RS01715 +1.0
Shikimate kinase I (EC 2.7.1.71) BWI76_RS26090 N.D.
Shikimate kinase II (EC 2.7.1.71) No genes
Shikimate kinase III (EC 2.7.1.71) BWI76_RS06185 -0.6
Shikimate/quinate 5-dehydrogenase I beta (EC 1.1.1.282) BWI76_RS04655 +0.8

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