SEED Subsystem: Isoleucine degradation in Pseudomonas fluorescens SBW25-INTG

Experiments:

Role Gene Name D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) PFLU_RS07650 -3.3
  PFLU_RS22840 +0.0
3-ketoacyl-CoA thiolase (EC 2.3.1.16) PFLU_RS14765 -0.1
  PFLU_RS16065 +0.1
  PFLU_RS21180 -0.0
3-ketoacyl-CoA thiolase [isoleucine degradation] (EC 2.3.1.16) No genes
Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2) No genes
Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12) No genes
Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) PFLU_RS19355 -0.2
Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4) PFLU_RS19360 -0.1
Branched-chain amino acid aminotransferase (EC 2.6.1.42) PFLU_RS19375 -0.3
Butyrate kinase (EC 2.7.2.7) No genes
Butyryl-CoA dehydrogenase (EC 1.3.99.2) PFLU_RS04175 +0.5
  PFLU_RS09195 -1.3
  PFLU_RS14770 -0.1
  PFLU_RS14780 +0.4
  PFLU_RS17205 -0.4
  PFLU_RS18005 +0.3
  PFLU_RS22410 +0.2
Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168) PFLU_RS19365 -0.2
Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4) PFLU_RS19370 +0.4
Enoyl-CoA hydratase (EC 4.2.1.17) PFLU_RS07105 +0.5
  PFLU_RS07650 -3.3
  PFLU_RS08485 +0.8
  PFLU_RS10835 -3.3
  PFLU_RS12025 +0.3
  PFLU_RS24260 +0.1
Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) PFLU_RS22840 +0.0
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.-) No genes
Predicted transcriptional regulator of leucine degradation pathway, MerR family No genes
Probable acyl-CoA dehydrogenase (EC 1.3.99.3) PFLU_RS14760 -0.7
  PFLU_RS16950 +0.0
Transcriptional regulator BkdR of isoleucine and valine catabolism operon PFLU_RS19350 -1.0
phosphotransbutyrylase (EC 2.3.1.19) No genes

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Or view this subsystem at the SEED (slow)