SEED Subsystem: Peptidoglycan Biosynthesis in Klebsiella michiganensis M5al

Experiments:

Role Gene Name RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x  
Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129) BWI76_RS04730 N.D.
  BWI76_RS13995 -0.5
  BWI76_RS17925 -0.1
D-alanine--D-alanine ligase (EC 6.3.2.4) BWI76_RS06125 +1.2
D-alanine--D-alanine ligase A (EC 6.3.2.4) No genes
D-alanine--D-alanine ligase B (EC 6.3.2.4) No genes
D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) BWI76_RS08020 -2.4
  BWI76_RS09145 -0.1
  BWI76_RS18780 -0.2
  BWI76_RS25165 +0.2
Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157) BWI76_RS00170 N.D.
Glutamate racemase (EC 5.1.1.3) BWI76_RS00895 N.D.
Glutamine synthetase type I (EC 6.3.1.2) BWI76_RS00400 -2.0
Glutamine synthetase type II, eukaryotic (EC 6.3.1.2) No genes
Glutamine synthetase type III, GlnN (EC 6.3.1.2) No genes
Glutamine synthetase, clostridia type (EC 6.3.1.2) No genes
Membrane-bound lytic murein transglycosylase B (EC 3.2.1.-) No genes
Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) BWI76_RS22210 +0.4
Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-) BWI76_RS25290 -0.4
Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) BWI76_RS05120 -1.7
  BWI76_RS26125 -0.0
Murein-DD-endopeptidase (EC 3.4.99.-) BWI76_RS19525 -0.1
N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) BWI76_RS00170 N.D.
N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23) No genes
Penicillin-binding protein 2 (PBP-2) BWI76_RS08035 N.D.
Penicillin-binding protein 4 precursor No genes
Penicillin-binding protein PBP2a, methicillin resistance determinant MecA, transpeptidase No genes
Penicillin-insensitive transglycosylase (EC 2.4.2.-) & transpeptidase PBP-1C BWI76_RS21105 -0.4
Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) BWI76_RS04745 N.D.
Putative glutamine synthetase, Rickettsiales type (EC 6.3.1.2) No genes
Rare lipoprotein A precursor BWI76_RS08025 -0.0
Rod shape-determining protein RodA BWI76_RS08030 N.D.
UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158) BWI76_RS01420 N.D.
UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) BWI76_RS25200 N.D.
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227) BWI76_RS04760 N.D.
UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8) BWI76_RS04765 N.D.
UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) BWI76_RS04750 N.D.
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13) BWI76_RS04735 N.D.
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10) BWI76_RS04740 N.D.

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