SEED Subsystem: Acetyl-CoA fermentation to Butyrate in Pseudomonas stutzeri RCH2

Experiments:

Role Gene Name L-Isoleucine (C)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) Psest_2654 N.D.
  Psest_3800 +0.0
  Psest_3969 -0.1
  Psest_4235 -0.1
3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) No genes
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) No genes
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) No genes
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) Psest_2654 N.D.
  Psest_3969 -0.1
  Psest_4235 -0.1
Acetoacetate metabolism regulatory protein AtoC No genes
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) Psest_1076 +0.3
Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8) No genes
Acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (EC 2.8.3.8) No genes
Butyrate kinase (EC 2.7.2.7) No genes
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
Butyryl-CoA dehydrogenase (EC 1.3.99.2) Psest_2440 +0.1
  Psest_2445 -3.2
  Psest_2475 -0.2
  Psest_2728 +0.0
  Psest_4233 +0.2
Electron transfer flavoprotein, alpha subunit Psest_1706 N.D.
Electron transfer flavoprotein, beta subunit Psest_1705 N.D.
Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) Psest_1704 -2.4
Enoyl-CoA hydratase (EC 4.2.1.17) Psest_2654 N.D.
  Psest_3969 -0.1
  Psest_4235 -0.1
Ferredoxin-like protein No genes
Ferredoxin-like protein FixX No genes
Ferredoxin-like protein YdiT No genes
Ferredoxin-like protein YgcO No genes
Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-) No genes
phosphotransbutyrylase (EC 2.3.1.19) No genes

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