SEED Subsystem: Cysteine Biosynthesis in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name RCH2 defined with Cobalt chloride 0.04 mM  
⨯
Adenylylsulfate kinase (EC 2.7.1.25) Pf6N2E2_3654 +0.1
  Pf6N2E2_5258 -4.4
Cys regulon transcriptional activator CysB Pf6N2E2_1337 +0.7
  Pf6N2E2_2465 -2.6
  Pf6N2E2_61 -0.1
  Pf6N2E2_62 -0.0
Cystathionine beta-synthase (EC 4.2.1.22) No genes
Cystathionine gamma-lyase (EC 4.4.1.1) Pf6N2E2_5896 +0.1
Cysteine synthase (EC 2.5.1.47) Pf6N2E2_2700 -0.8
  Pf6N2E2_2936 +0.2
Cysteine synthase A (EC 2.5.1.47) No genes
Cysteine synthase B (EC 2.5.1.47) Pf6N2E2_5721 N.D.
Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) Pf6N2E2_6054 N.D.
Serine acetyltransferase (EC 2.3.1.30) Pf6N2E2_3191 +5.3
Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) Pf6N2E2_5258 -4.4
Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) Pf6N2E2_5257 -2.9
Sulfate and thiosulfate binding protein CysP Pf6N2E2_4006 -0.2
  Pf6N2E2_5832 +0.5
Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25) Pf6N2E2_4002 +0.3
Sulfate permease Pf6N2E2_3443 +0.1
  Pf6N2E2_4131 -0.2
Sulfate permease, Trk-type No genes
Sulfate transport system permease protein CysT Pf6N2E2_4004 +0.5
Sulfate transport system permease protein CysW Pf6N2E2_4003 +0.4
Sulfate transporter, CysZ-type Pf6N2E2_5267 -0.1
Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2) Pf6N2E2_1511 -0.3
Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) Pf6N2E2_2073 -4.1

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