MetaCyc Pathway: even iso-branched-chain fatty acid biosynthesis in Pseudomonas fluorescens FW300-N2E2

Add experiment(s):


Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)
Pf6N2E2_477
2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD+→isobutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
isobutanoyl-CoA + a malonyl-[acp] + H+→a 4-methyl-3-oxopentanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.300)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 4-methyl-3-oxopentanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-4-methylpentanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-4-methylpentanoyl-[acp]→a (2E)-4-methylpent-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-4-methylpent-2-enoyl-[acp] + NADH + H+→a 5-methylhexanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-4-methylpent-2-enoyl-[acp] + NADPH + H+→a 5-methylhexanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 5-methylhexanoyl-[acp] + a malonyl-[acp] + H+→a 6-methyl-3-oxoheptanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 6-methyl-3-oxoheptanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-6-methylheptanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-6-methylheptanoyl-[acp]→a (2E)-6-methylhept-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-6-methylhept-2-enoyl-[acp] + NADH + H+→a 6-methylheptanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-6-methylhept-2-enoyl-[acp] + NADPH + H+→a 6-methylheptanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 6-methylheptanoyl-[acp] + a malonyl-[acp] + H+→an 8-methyl-3-oxononanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an 8-methyl-3-oxononanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-8-methylnonanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-8-methylnonanoyl-[acp]→a (2E)-8-methylnon-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-8-methylnon-2-enoyl-[acp] + NADH + H+→an 8-methylnonanoyl-[acyl-carrier protein] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-8-methylnon-2-enoyl-[acp] + NADPH + H+→an 8-methylnonanoyl-[acyl-carrier protein] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
an 8-methylnonanoyl-[acyl-carrier protein] + a malonyl-[acp] + H+→a 1o-methyl-3-oxoundecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 1o-methyl-3-oxoundecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-10-methylundecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-10-methylundecanoyl-[acp]→a (2E)-10-methylundec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-10-methylundec-2-enoyl-[acp] + NADH + H+→an 10-methylundecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-10-methylundec-2-enoyl-[acp] + NADPH + H+→an 10-methylundecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
an 10-methylundecanoyl-[acp] + a malonyl-[acp] + H+→a 12-methyl-3-oxotridecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 12-methyl-3-oxotridecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-12-methyltridecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-12-methyltridecanoyl-[acp]→a (2E)-12-methyltridec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-12-methyltridec-2-enoyl-[acp] + NADH + H+→a 12-methyltridecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-12-methyltridec-2-enoyl-[acp] + NADPH + H+→a 12-methyltridecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 12-methyltridecanoyl-[acp] + a malonyl-[acp] + H+→a 14-methyl-3-oxopentadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
Oleoyl-[acyl-carrier-protein] hydrolase:
a 12-methyltridecanoyl-[acp] + H2O→12-methyltridecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 14-methyl-3-oxopentadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-14-methylpentadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-14-methylpentadecanoyl-[acp]→a (2E)-14-methylpentadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-14-methylpentadec-2-enoyl-[acp] + NADH + H+→a 14-methylpentadecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-14-methylpentadec-2-enoyl-[acp] + NADPH + H+→a 14-methylpentadecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.39)
Pf6N2E2_1446
Oleoyl-[acyl-carrier-protein] hydrolase:
a 14-methylpentadecanoyl-[acp] + H2O→14-methylpentadecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information