MetaCyc Pathway: odd iso-branched-chain fatty acid biosynthesis in Pseudomonas fluorescens FW300-N2E2

Add experiment(s):


Branched-chain-amino-acid transaminase:
L-leucine + 2-oxoglutarate→4-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42; 2.6.1.6)
Pf6N2E2_477
2-oxoisovalerate dehydrogenase (acylating):
4-methyl-2-oxopentanoate + coenzyme A + NAD+→3-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
3-methylbutanoyl-CoA + a malonyl-[acp] + H+→a 5-methyl-3-oxohexanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.300)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 5-methyl-3-oxohexanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-5-methylhexanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-5-methylhexanoyl-[acp]→a (2E)-5-methylhex-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-5-methylhex-2-enoyl-[acp] + NADH + H+→a 5-methylhexanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-5-methylhex-2-enoyl-[acp] + NADPH + H+→a 5-methylhexanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 5-methylhexanoyl-[acp] + a malonyl-[acp] + H+→a 7-methyl-3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 7-methyl-3-oxooctanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-7-methyloctanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-7-methyloctanoyl-[acp]→a (2E)-7-methyloct-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-7-methyloct-2-enoyl-[acp] + NADH + H+→a 7-methyloctanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-7-methyloct-2-enoyl-[acp] + NADPH + H+→a 7-methyloctanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 7-methyloctanoyl-[acp] + a malonyl-[acp] + H+→a 9-methyl-3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 9-methyl-3-oxodecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-9-methyldecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-9-methyldecanoyl-[acp]→a (2E)-9-methyldec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-9-methyldec-2-enoyl-[acp] + NADH + H+→a 9-methyldecanoyl-[acyl-carrier protein] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-9-methyldec-2-enoyl-[acp] + NADPH + H+→a 9-methyldecanoyl-[acyl-carrier protein] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 9-methyldecanoyl-[acyl-carrier protein] + a malonyl-[acp] + H+→a 11-methyl-3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 11-methyl-3-oxododecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-11-methyldodecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-11-methyldodecanoyl-[acp]→a (2E)-11-methyldodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-11-methyldodec-2-enoyl-[acp] + NADH + H+→an 11-methyldodecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-11-methyldodec-2-enoyl-[acp] + NADPH + H+→an 11-methyldodecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
an 11-methyldodecanoyl-[acp] + a malonyl-[acp] + H+→a 13-methyl-3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 13-methyl-3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-13-methyltetradecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-13-methyltetradecanoyl-[acp]→a (2E)-13-methyltetradec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-13-methyltetradec-2-enoyl-[acp] + NADH + H+→a 13-methyltetradecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-13-methyltetradec-2-enoyl-[acp] + NADPH + H+→a 13-methyltetradecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
Pf6N2E2_1446
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 13-methyltetradecanoyl-[acp] + a malonyl-[acp] + H+→a 15-methyl-3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Pf6N2E2_3729
Pf6N2E2_5954
Pf6N2E2_835
Oleoyl-[acyl-carrier-protein] hydrolase:
a 13-methyltetradecanoyl-[acp] + H2O→13-methyltetradecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 15-methyl-3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-15-methylhexadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004
Pf6N2E2_1181
Pf6N2E2_1257
Pf6N2E2_1323
Pf6N2E2_1747
Pf6N2E2_1925
Pf6N2E2_1928
Pf6N2E2_3493
Pf6N2E2_3704
Pf6N2E2_3728
Pf6N2E2_383
Pf6N2E2_3916
Pf6N2E2_394
Pf6N2E2_4183
Pf6N2E2_5206
Pf6N2E2_5209
Pf6N2E2_5603
Pf6N2E2_5952
Pf6N2E2_5967
Pf6N2E2_6050
Pf6N2E2_686
Pf6N2E2_853
Pf6N2E2_990
Pf6N2E2_995
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-15-methylhexadecanoyl-[acp]→a (2E)-15-methylhexadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058
Pf6N2E2_5871
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-15-methylhexadec-2-enoyl-[acp] + NADH + H+→a 15-methylhexadecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433
Pf6N2E2_580
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-15-methylhexadec-2-enoyl-[acp] + NADPH + H+→a 15-methylhexadecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Pf6N2E2_1446
Oleoyl-[acyl-carrier-protein] hydrolase:
a 15-methylhexadecanoyl-[acp] + H2O→15-methylhexadecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information