MetaCyc Pathway: L-glutamate degradation V (via hydroxyglutarate) in Pseudomonas fluorescens FW300-N2E2

Add experiment(s):


Glutamate dehydrogenase:
L-glutamate + NAD+ + H2O→2-oxoglutarate + ammonium + NADH + H+ (EC 1.4.1.2)
Pf6N2E2_634
2-oxoglutarate + NADH + H+→(R)-2-hydroxyglutarate + NAD+ (EC 1.1.1.399)
Pf6N2E2_3932
Glutaconate CoA-transferase:
(R)-2-hydroxyglutarate + acetyl-CoA→(R)-2-hydroxyglutaryl-CoA + acetate (EC 2.8.3.12)
Pf6N2E2_2836
Pf6N2E2_2837
(R)-2-hydroxyglutaryl-CoA→(E)-glutaconyl-CoA + H2O (EC 4.2.1.167)
No genes
(E)-glutaconyl-CoA[in] + Na+[in] + H+[in]→crotonyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.5)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
Pf6N2E2_1013
Pf6N2E2_1046
Pf6N2E2_1834
Pf6N2E2_1835
Pf6N2E2_1840
Pf6N2E2_1922
Pf6N2E2_1934
Pf6N2E2_1935
Pf6N2E2_2290
Pf6N2E2_2773
Pf6N2E2_3894
Pf6N2E2_5493
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase:
butanoyl-CoA + acetate→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
Pf6N2E2_1722
Pf6N2E2_1833
Pf6N2E2_1850
Pf6N2E2_1922
Pf6N2E2_2290
Pf6N2E2_3894
Pf6N2E2_4690
Pf6N2E2_6000
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
Pf6N2E2_1145
Pf6N2E2_1675
Pf6N2E2_1836
Pf6N2E2_1851
Pf6N2E2_1923
Pf6N2E2_2113
Pf6N2E2_2289
Pf6N2E2_2539
Pf6N2E2_2835
Pf6N2E2_3492
Pf6N2E2_5999

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information