MetaCyc Pathway: L-glutamate degradation VII (to butanoate) in Pseudomonas fluorescens FW300-N2E2

Add experiment(s):


Methylaspartate mutase (in reverse):
L-glutamate→(2S, 3S)-3-methylaspartate (EC 5.4.99.1)
No genes
Methylaspartate ammonia-lyase:
(2S, 3S)-3-methylaspartate→mesaconate + ammonium (EC 4.3.1.2)
No genes
(S)-2-methylmalate dehydratase (in reverse):
mesaconate + H2O→(S)-citramalate (EC 4.2.1.34)
No genes
Citramalate lyase:
(S)-citramalate→pyruvate + acetate (EC 4.1.3.22)
No genes
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + 2 a reduced ferredoxin [iron-sulfur] cluster + CO2 + H+ (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Pf6N2E2_1145
Pf6N2E2_1675
Pf6N2E2_1836
Pf6N2E2_1851
Pf6N2E2_1923
Pf6N2E2_2113
Pf6N2E2_2289
Pf6N2E2_2539
Pf6N2E2_2835
Pf6N2E2_3492
Pf6N2E2_5999
Ferredoxin hydrogenase (in reverse):
2 a reduced ferredoxin [iron-sulfur] cluster + 2 H+→H2 + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.12.7.2)
No genes
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
Pf6N2E2_1722
Pf6N2E2_1833
Pf6N2E2_1850
Pf6N2E2_1922
Pf6N2E2_2290
Pf6N2E2_3894
Pf6N2E2_4690
Pf6N2E2_6000
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
Pf6N2E2_1013
Pf6N2E2_1046
Pf6N2E2_1834
Pf6N2E2_1835
Pf6N2E2_1840
Pf6N2E2_1922
Pf6N2E2_1934
Pf6N2E2_1935
Pf6N2E2_2290
Pf6N2E2_2773
Pf6N2E2_3894
Pf6N2E2_5493
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] + butanoyl-CoA[cytosol] + 2 NAD+[cytosol] (EC 1.3.1.109)
No genes
Phosphate butyryltransferase:
butanoyl-CoA + phosphate→butanoyl phosphate + coenzyme A (EC 2.3.1.19)
No genes
Branched-chain-fatty-acid kinase (in reverse):
butanoyl phosphate + ADP→butanoate + ATP (EC 2.7.2.14; 2.7.2.7)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information