MetaCyc Pathway: L-tryptophan biosynthesis in Pseudomonas fluorescens FW300-N2C3

Anthranilate synthase: chorismate + L-glutamine→anthranilate + L-glutamate + H+ + pyruvate (EC 4.1.3.27)

AO356_14420

AO356_14430

Anthranilate phosphoribosyltransferase (in reverse): anthranilate + 5-phospho-α-D-ribose 1-diphosphate→N-(5-phosphoribosyl)-anthranilate + diphosphate (EC 2.4.2.18)

AO356_14435

AO356_22210

Phosphoribosylanthranilate isomerase: N-(5-phosphoribosyl)-anthranilate→1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate (EC 5.3.1.24)

AO356_21440

Indole-3-glycerol-phosphate synthase: 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H+→(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O (EC 4.1.1.48)

AO356_14440

Indole-3-glycerol-phosphate lyase: (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate→indole + D-glyceraldehyde 3-phosphate (EC 4.1.2.8; 4.2.1.20)

AO356_10575

AO356_10910

AO356_10915

AO356_16575

indole + L-serine→L-tryptophan + H2O (EC 4.2.1.122; 4.2.1.20)

AO356_10575

AO356_10910

AO356_10915

AO356_16575

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information