MetaCyc Pathway: (5Z)-dodecenoate biosynthesis II in Pseudomonas fluorescens FW300-N2C3

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Acyl-CoA oxidase:
a decanoyl-[acp] + dioxygen→a (2E)-dec-2-enoyl-[acp] + hydrogen peroxide (EC 1.3.3.6)
No genes
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dec-2-enoyl-[acp]→a (3Z)-dec-3-enoyl-[acp] (EC 5.3.3.14)
AO356_19775
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxododec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
AO356_09210
AO356_09225
AO356_19770
AO356_20175
AO356_26850
AO356_27195
AO356_27550
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxododec-5-enoyl-[acp] + NADPH + H+→a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp] + NADP+ (EC 1.1.1.100)
AO356_08105
AO356_09105
AO356_09220
AO356_10220
AO356_11565
AO356_16330
AO356_18385
AO356_20165
AO356_20240
AO356_20810
AO356_22455
AO356_22525
AO356_24985
AO356_25515
AO356_26870
AO356_27315
AO356_28680
AO356_30325
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp]→a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + H2O (EC 4.2.1.59)
AO356_05985
AO356_19775
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + NADH + H+→a (5Z)-dodec-5-enoyl-[acp] + NAD+ (EC 1.3.1.9)
AO356_22745
AO356_24445

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information