MetaCyc Pathway: benzoate fermentation (to acetate and cyclohexane carboxylate) in Pseudomonas fluorescens FW300-N2C3

Add experiment(s):


Benzoate--CoA ligase:
benzoate + ATP + coenzyme A→benzoyl-CoA + AMP + diphosphate (EC 6.2.1.25)
No genes
benzoyl-CoA + 2 ATP + 2 a reduced ferredoxin [iron-sulfur] cluster + 2 H2O→cyclohex-1,5-diene-1-carbonyl-CoA + 2 ADP + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 phosphate (EC 1.3.7.8)
No genes
Cyclohexa-1,5-dienecarbonyl-CoA hydratase (in reverse):
cyclohex-1,5-diene-1-carbonyl-CoA + H2O→6-hydroxycyclohex-1-ene-1-carbonyl-CoA (EC 4.2.1.100)
No genes
cyclohex-1,5-diene-1-carbonyl-CoA + a reduced electron-transfer flavoprotein→cyclohex-1-ene-1-carbonyl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.10)
No genes
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+→6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ (EC 1.1.1.368)
No genes
cyclohex-1-ene-1-carbonyl-CoA + a reduced electron-transfer flavoprotein→cyclohexane-1-carbonyl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.11)
No genes
cyclohexane-1-carbonyl-CoA + H2O→cyclohexane-1-carboxylate + coenzyme A + H+
No genes
6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O→2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA (EC 3.7.1.21)
No genes
2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H2O→3-hydroxypimeloyl-CoA + H+ (EC 3.7.1.21)
No genes
3-hydroxypimeloyl-CoA dehydrogenase:
3-hydroxypimeloyl-CoA + NAD+→3-oxopimeloyl-CoA + NADH + H+ (EC 1.1.1.259)
No genes
3-oxopimeloyl-CoA + coenzyme A→glutaryl-CoA + acetyl-CoA
AO356_03560
glutaryl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-glutaconyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.6)
No genes
(E)-glutaconyl-CoA + H+→crotonyl-CoA + CO2 (EC 1.3.8.6)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
AO356_02330
AO356_04640
AO356_10120
AO356_12440
AO356_17825
AO356_26645
AO356_30295
AO356_30355
AO356_30360
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
AO356_02330
AO356_10120
AO356_13845
AO356_20400
AO356_29995
AO356_30295
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
AO356_02325
AO356_03560
AO356_04930
AO356_08090
AO356_20395
AO356_21640
AO356_26350
AO356_30300
Acetate--CoA ligase (ADP-forming) (in reverse):
acetyl-CoA + ADP + phosphate→acetate + ATP + coenzyme A (EC 6.2.1.13)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information