MetaCyc Pathway: protocatechuate degradation II (ortho-cleavage pathway) in Pseudomonas fluorescens FW300-N2C3

Protocatechuate 3,4-dioxygenase:
protocatechuate + dioxygen→3-carboxy-cis,cis-muconate + 2 H⁺ (EC 1.13.11.3)

3-carboxy-cis,cis-muconate cycloisomerase (in reverse):
3-carboxy-cis,cis-muconate + H⁺→(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate (EC 5.5.1.2)

AO356_04910

4-carboxymuconolactone decarboxylase:
(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate + H⁺→(4,5-dihydro-5-oxofuran-2-yl)-acetate + CO₂ (EC 4.1.1.44)

3-oxoadipate enol-lactonase:
(4,5-dihydro-5-oxofuran-2-yl)-acetate + H₂O→3-oxoadipate + H⁺ (EC 3.1.1.24)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information