MetaCyc Pathway: mixed acid fermentation in Pseudomonas fluorescens FW300-N2C3

MetaCyc Pathway: mixed acid fermentation in Pseudomonas fluorescens FW300-N2C3

Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)

Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H⁺→pyruvate + ATP (EC 2.7.1.40)

D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H⁺→(R)-lactate + NAD⁺ (EC 1.1.1.28)

Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H⁺→(S)-malate + NAD⁺ (EC 1.1.1.37; 1.1.1.38)

No genes

Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)

No genes

Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H⁺→acetaldehyde + coenzyme A + NAD⁺ (EC 1.2.1.10)

Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H₂O→citrate + coenzyme A + H⁺ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)

formate + H⁺→CO₂ + H₂

No genes

Fumarate hydratase:
(S)-malate→fumarate + H₂O (EC 4.2.1.2)

Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)

Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)

Aconitate hydratase:
citrate→cis-aconitate + H₂O (EC 4.2.1.3)

Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H⁺→ethanol + NAD⁺ (EC 1.1.1.1)

Succinate dehydrogenase (ubiquinone):
fumarate_[in] + a menaquinol→succinate_[in] + a menaquinone (EC 1.3.5.1)

No genes

Aconitate hydratase:
cis-aconitate + H₂O→D-threo-isocitrate (EC 4.2.1.3)

Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP⁺→CO₂ + 2-oxoglutarate + NADPH (EC 1.1.1.42)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information