MetaCyc Pathway: pyruvate fermentation to butanol II (engineered) in Pseudomonas fluorescens FW300-N2E3

Add experiment(s):


Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
AO353_00505
AO353_01065
AO353_01160
AO353_05785
AO353_13880
AO353_17205
AO353_25685
AO353_27530
AO353_27945
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
AO353_00510
AO353_01155
AO353_27535
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
AO353_00510
AO353_03780
AO353_17555
AO353_19125
AO353_23055
AO353_24830
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
AO353_00670
AO353_01500
AO353_15135
AO353_15225
AO353_24835
AO353_25670
AO353_25680
AO353_28600
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information