MetaCyc Pathway: glutaminyl-tRNAgln biosynthesis via transamidation in Pseudomonas fluorescens FW300-N2E3

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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
AO353_00925
AO353_01150
AO353_02570
AO353_03130
AO353_03905
AO353_04395
AO353_05255
AO353_05260
AO353_05265
AO353_05630
AO353_05635
AO353_06730
AO353_07220
AO353_07230
AO353_10550
AO353_12515
AO353_12520
AO353_15955
AO353_16160
AO353_16550
AO353_17090
AO353_17175
AO353_19025
AO353_20130
AO353_20805
AO353_26575
AO353_26790
Glutamate--tRNA(Gln) ligase:
L-glutamate + ATP + a tRNAGln→an L-glutamyl-[tRNAGln] + AMP + diphosphate (EC 6.1.1.24)
AO353_20560
Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
an L-glutamyl-[tRNAGln] + ATP→a 5-phosphooxy-L-glutamyl-[tRNAGln] + ADP (EC 6.3.5.7)
AO353_05255
AO353_05260
AO353_05265
Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
a 5-phosphooxy-L-glutamyl-[tRNAGln] + ammonia→an L-glutaminyl-[tRNAGln] + phosphate (EC 6.3.5.7)
AO353_05255
AO353_05260
AO353_05265

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information