MetaCyc Pathway: L-glutamate degradation V (via hydroxyglutarate) in Pseudomonas fluorescens FW300-N2E3

Add experiment(s):


Glutamate dehydrogenase:
L-glutamate + NAD+ + H2O→2-oxoglutarate + ammonium + NADH + H+ (EC 1.4.1.2)
AO353_22820
2-oxoglutarate + NADH + H+→(R)-2-hydroxyglutarate + NAD+ (EC 1.1.1.399)
AO353_08510
Glutaconate CoA-transferase:
(R)-2-hydroxyglutarate + acetyl-CoA→(R)-2-hydroxyglutaryl-CoA + acetate (EC 2.8.3.12)
AO353_17195
AO353_17200
(R)-2-hydroxyglutaryl-CoA→(E)-glutaconyl-CoA + H2O (EC 4.2.1.167)
No genes
(E)-glutaconyl-CoA[in] + Na+[in] + H+[in]→crotonyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.5)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
AO353_00510
AO353_03780
AO353_17555
AO353_19125
AO353_23055
AO353_24830
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase:
butanoyl-CoA + acetate→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
AO353_00510
AO353_01155
AO353_27535
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
AO353_00505
AO353_01065
AO353_01160
AO353_05785
AO353_13880
AO353_17205
AO353_25685
AO353_27530
AO353_27945

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information