MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Pseudomonas fluorescens FW300-N1B4

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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
Pf1N1B4_1100
Pf1N1B4_1101
Pf1N1B4_1102
Pf1N1B4_1172
Pf1N1B4_1173
Pf1N1B4_1533
Pf1N1B4_1534
Pf1N1B4_2545
Pf1N1B4_2547
Pf1N1B4_2646
Pf1N1B4_290
Pf1N1B4_3102
Pf1N1B4_3209
Pf1N1B4_3229
Pf1N1B4_3412
Pf1N1B4_3592
Pf1N1B4_3722
Pf1N1B4_374
Pf1N1B4_3797
Pf1N1B4_3814
Pf1N1B4_3940
Pf1N1B4_4165
Pf1N1B4_4436
Pf1N1B4_4516
Pf1N1B4_6037
Pf1N1B4_706
Pf1N1B4_839
Pf1N1B4_875
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23)
Pf1N1B4_641
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6)
Pf1N1B4_1100
Pf1N1B4_1101
Pf1N1B4_1102
Pf1N1B4_4165
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6)
Pf1N1B4_1100
Pf1N1B4_1101
Pf1N1B4_1102
Pf1N1B4_4165

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information