MetaCyc Pathway: butanol and isobutanol biosynthesis (engineered) in Pseudomonas fluorescens FW300-N1B4

Add experiment(s):


Glycine oxidase:
glycine + dioxygen + H2O→glyoxylate + ammonium + hydrogen peroxide (EC 1.4.3.19; 1.4.3.3)
Pf1N1B4_2817
Pf1N1B4_325
Pf1N1B4_5965
3-ethylmalate synthase:
glyoxylate + butanoyl-CoA + H2O→3-ethylmalate + coenzyme A + H+ (EC 2.3.3.7)
No genes
3-isopropylmalate dehydrogenase:
3-ethylmalate + NAD+→2-oxovalerate + CO2 + NADH (EC 1.1.1.85)
Pf1N1B4_12
2-oxovalerate→3-methyl-2-oxobutanoate
No genes
Pyruvate decarboxylase:
2-oxovalerate + H+→1-butanal + CO2 (EC 4.1.1.1)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
Pf1N1B4_2542
Pf1N1B4_3108
Pf1N1B4_3270
Pf1N1B4_395
Pf1N1B4_4176
Pf1N1B4_4243
Pf1N1B4_4506
Pf1N1B4_4898
Pf1N1B4_5026

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information