MetaCyc Pathway: heterolactic fermentation in Pseudomonas fluorescens FW300-N1B4

Add experiment(s):


Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H+ (EC 2.7.1.4)
Pf1N1B4_4844
Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H+ (EC 2.7.1.1; 2.7.1.2)
Pf1N1B4_600
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
Pf1N1B4_2850
Glucose-6-phosphate dehydrogenase:
D-glucopyranose 6-phosphate + NADP+→6-phospho D-glucono-1,5-lactone + NADPH + H+ (EC 1.1.1.49)
Pf1N1B4_4726
Pf1N1B4_589
6-phosphogluconolactonase:
6-phospho D-glucono-1,5-lactone + H2O→D-gluconate 6-phosphate + H+ (EC 3.1.1.31)
Pf1N1B4_588
D-gluconate 6-phosphate + NAD(P)+→CO2 + D-ribulose 5-phosphate + NAD(P)H (EC 1.1.1.351)
No genes
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
Pf1N1B4_2543
Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O (EC 4.1.2.9)
No genes
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
Pf1N1B4_3917
Pf1N1B4_5956
Pf1N1B4_602
Phosphate acetyltransferase (in reverse):
acetyl phosphate + coenzyme A→acetyl-CoA + phosphate (EC 2.3.1.8)
Pf1N1B4_1054
Pf1N1B4_1055
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
Pf1N1B4_4506
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
Pf1N1B4_2399
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
Pf1N1B4_2542
Pf1N1B4_3108
Pf1N1B4_3270
Pf1N1B4_395
Pf1N1B4_4176
Pf1N1B4_4243
Pf1N1B4_4506
Pf1N1B4_4898
Pf1N1B4_5026
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
No genes
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
Pf1N1B4_3104
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
Pf1N1B4_3657
Pf1N1B4_678
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
Pf1N1B4_3500
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
Pf1N1B4_1188

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information