MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Pseudomonas stutzeri RCH2

MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Pseudomonas stutzeri RCH2

Glutamate dehydrogenase (NAD(P)(+)) (in reverse):
2-oxoglutarate + ammonium + NAD(P)H + H⁺→L-glutamate + NAD(P)⁺ + H₂O (EC 1.4.1.3)

No genes

Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H⁺ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)

No genes

Glutamate synthase (NADH) (in reverse):
2-oxoglutarate + L-glutamine + NADH + H⁺→2 L-glutamate + NAD⁺ (EC 1.4.1.14)

Glutamate synthase (NADPH):
L-glutamine + H₂O→L-glutamate + ammonia + H⁺ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)

Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H⁺ + phosphate (EC 6.3.1.2)

Glutamate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + ammonium + NADPH + H⁺→L-glutamate + NADP⁺ + H₂O (EC 1.4.1.4)

Glutamate synthase (NADPH) (in reverse):
2-oxoglutarate + ammonia + NADPH + 2 H⁺→L-glutamate + NADP⁺ + H₂O (EC 1.4.1.13; 1.4.1.14)

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Pathway details at MetaCyc
Fitness data for 25 genes
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All MetaCyc pathways for Pseudomonas stutzeri RCH2

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information