MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Pseudomonas stutzeri RCH2

MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Pseudomonas stutzeri RCH2

Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD⁺→β-D-fructofuranose 6-phosphate + NADH + H⁺ (EC 1.1.1.17)

No genes

Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD⁺→keto-D-fructose 6-phosphate + NADH + H⁺ (EC 1.1.1.140)

6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H⁺ (EC 2.7.1.11)

Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)

Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H₂O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)

Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)

Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD⁺ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H⁺ (EC 1.2.1.12)

Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)

3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)

3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)

No genes

Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H₂O (EC 4.2.1.11)

Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H⁺→pyruvate + ATP (EC 2.7.1.40)

L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H⁺→(S)-lactate + NAD⁺ (EC 1.1.1.27)

Pyruvate, water dikinase:
pyruvate + ATP + H₂O→phosphoenolpyruvate + AMP + 2 H⁺ + phosphate (EC 2.7.9.2)

Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)

No genes

Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H⁺→acetaldehyde + coenzyme A + NAD⁺ (EC 1.2.1.10)

Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)

Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)

Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H⁺→ethanol + NAD⁺ (EC 1.1.1.1)

Links:

Pathway details at MetaCyc
Fitness data for 30 genes
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All MetaCyc pathways for Pseudomonas stutzeri RCH2

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information