MetaCyc Pathway: mixed acid fermentation in Azospirillum brasilense Sp245

Add experiment(s):


Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
No genes
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
AZOBR_RS06845
AZOBR_RS21415
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
No genes
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
AZOBR_RS14060
AZOBR_RS16020
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
AZOBR_RS21180
AZOBR_RS30150
AZOBR_RS30195
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
AZOBR_RS32240
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
AZOBR_RS25925
formate + H+→CO2 + H2
AZOBR_RS14525
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
AZOBR_RS06120
AZOBR_RS10315
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
AZOBR_RS04600
AZOBR_RS12500
AZOBR_RS32455
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
AZOBR_RS04605
AZOBR_RS12495
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
AZOBR_RS02985
AZOBR_RS17090
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
AZOBR_RS06065
AZOBR_RS10415
AZOBR_RS13230
AZOBR_RS16515
AZOBR_RS20975
AZOBR_RS26265
AZOBR_RS26520
AZOBR_RS27605
AZOBR_RS28580
AZOBR_RS32240
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
AZOBR_RS02985
AZOBR_RS17090
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
AZOBR_RS10995
AZOBR_RS11025

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information