MetaCyc Pathway: odd <i>iso</i>-branched-chain fatty acid biosynthesis in Acidovorax sp. GW101-3H11

MetaCyc Pathway: odd iso-branched-chain fatty acid biosynthesis in Acidovorax sp. GW101-3H11

Branched-chain-amino-acid transaminase:
L-leucine + 2-oxoglutarate→4-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42; 2.6.1.6)

2-oxoisovalerate dehydrogenase (acylating):
4-methyl-2-oxopentanoate + coenzyme A + NAD⁺→3-methylbutanoyl-CoA + CO₂ + NADH (EC 1.2.1.25)

No genes

3-methylbutanoyl-CoA + a malonyl-[acp] + H⁺→a 5-methyl-3-oxohexanoyl-[acp] + CO₂ + coenzyme A (EC 2.3.1.300)

No genes

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 5-methyl-3-oxohexanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-5-methylhexanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-5-methylhexanoyl-[acp]→a (2E)-5-methylhex-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-5-methylhex-2-enoyl-[acp] + NADH + H⁺→a 5-methylhexanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-5-methylhex-2-enoyl-[acp] + NADPH + H⁺→a 5-methylhexanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a 5-methylhexanoyl-[acp] + a malonyl-[acp] + H⁺→a 7-methyl-3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 7-methyl-3-oxooctanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-7-methyloctanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-7-methyloctanoyl-[acp]→a (2E)-7-methyloct-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-7-methyloct-2-enoyl-[acp] + NADH + H⁺→a 7-methyloctanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-7-methyloct-2-enoyl-[acp] + NADPH + H⁺→a 7-methyloctanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a 7-methyloctanoyl-[acp] + a malonyl-[acp] + H⁺→a 9-methyl-3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 9-methyl-3-oxodecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-9-methyldecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-9-methyldecanoyl-[acp]→a (2E)-9-methyldec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-9-methyldec-2-enoyl-[acp] + NADH + H⁺→a 9-methyldecanoyl-[acyl-carrier protein] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-9-methyldec-2-enoyl-[acp] + NADPH + H⁺→a 9-methyldecanoyl-[acyl-carrier protein] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a 9-methyldecanoyl-[acyl-carrier protein] + a malonyl-[acp] + H⁺→a 11-methyl-3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 11-methyl-3-oxododecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-11-methyldodecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-11-methyldodecanoyl-[acp]→a (2E)-11-methyldodec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-11-methyldodec-2-enoyl-[acp] + NADH + H⁺→an 11-methyldodecanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-11-methyldodec-2-enoyl-[acp] + NADPH + H⁺→an 11-methyldodecanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Beta-ketoacyl-acyl-carrier-protein synthase II:
an 11-methyldodecanoyl-[acp] + a malonyl-[acp] + H⁺→a 13-methyl-3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 13-methyl-3-oxotetradecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-13-methyltetradecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-13-methyltetradecanoyl-[acp]→a (2E)-13-methyltetradec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-13-methyltetradec-2-enoyl-[acp] + NADH + H⁺→a 13-methyltetradecanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-13-methyltetradec-2-enoyl-[acp] + NADPH + H⁺→a 13-methyltetradecanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a 13-methyltetradecanoyl-[acp] + a malonyl-[acp] + H⁺→a 15-methyl-3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

Oleoyl-[acyl-carrier-protein] hydrolase:
a 13-methyltetradecanoyl-[acp] + H₂O→13-methyltetradecanoate + a soluble [acyl-carrier protein] + H⁺ (EC 3.1.2.14)

No genes

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 15-methyl-3-oxohexadecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-15-methylhexadecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-15-methylhexadecanoyl-[acp]→a (2E)-15-methylhexadec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-15-methylhexadec-2-enoyl-[acp] + NADH + H⁺→a 15-methylhexadecanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-15-methylhexadec-2-enoyl-[acp] + NADPH + H⁺→a 15-methylhexadecanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Oleoyl-[acyl-carrier-protein] hydrolase:
a 15-methylhexadecanoyl-[acp] + H₂O→15-methylhexadecanoate + a soluble [acyl-carrier protein] + H⁺ (EC 3.1.2.14)

No genes

Links:

Pathway details at MetaCyc
Fitness data for 26 genes
Select organism:
All MetaCyc pathways for Acidovorax sp. GW101-3H11

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information