MetaCyc Pathway: biotin biosynthesis I in Xanthomonas campestris pv. campestris strain 8004

a malonyl-[acp] + S-adenosyl-L-methionine→a malonyl-[acp] methyl ester + S-adenosyl-L-homocysteine (EC 2.1.1.197)

No genes

Beta-ketoacyl-acyl-carrier-protein synthase II: a malonyl-[acp] + a malonyl-[acp] methyl ester + H+→a 3-oxoglutaryl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)

Xcc-8004.2095.1

Xcc-8004.3993.1

Xcc-8004.3998.1

Xcc-8004.4537.1

Xcc-8004.5074.1

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse): a 3-oxoglutaryl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxyglutaryl-[acp] methyl ester + NADP+ (EC 1.1.1.100)

Xcc-8004.3996.1

Xcc-8004.4609.1

Xcc-8004.500.1

Xcc-8004.5082.1

Xcc-8004.551.1

Xcc-8004.558.1

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase: a (3R)-3-hydroxyglutaryl-[acp] methyl ester→a (2E)-enoylglutaryl-[acp] methyl ester + H2O (EC 4.2.1.59)

Xcc-8004.3567.1

Xcc-8004.4536.1

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse): a (2E)-enoylglutaryl-[acp] methyl ester + NADH + H+→a glutaryl-[acp] methyl ester + NAD+ (EC 1.3.1.9)

No genes

Beta-ketoacyl-acyl-carrier-protein synthase II: a glutaryl-[acp] methyl ester + a malonyl-[acp] + H+→a 3-oxopimeloyl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)

Xcc-8004.2095.1

Xcc-8004.3993.1

Xcc-8004.3998.1

Xcc-8004.4537.1

Xcc-8004.5074.1

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse): a 3-oxopimeloyl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxypimeloyl-[acp] methyl ester + NADP+ (EC 1.1.1.100)

Xcc-8004.3996.1

Xcc-8004.4609.1

Xcc-8004.500.1

Xcc-8004.5082.1

Xcc-8004.551.1

Xcc-8004.558.1

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase: a (3R)-3-hydroxypimeloyl-[acp] methyl ester→a (2E)-enoylpimeloyl-[acp] methyl ester + H2O (EC 4.2.1.59)

Xcc-8004.3567.1

Xcc-8004.4536.1

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse): a (2E)-enoylpimeloyl-[acp] methyl ester + NADH + H+→a pimeloyl-[acp] methyl ester + NAD+ (EC 1.3.1.9)

No genes

a pimeloyl-[acp] methyl ester + H2O→a pimeloyl-[acp] + methanol + H+ (EC 3.1.1.85)

Xcc-8004.502.1

8-amino-7-oxononanoate synthase: a pimeloyl-[acp] + L-alanine + H+→(8S)-8-amino-7-oxononanoate + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.47)

Xcc-8004.504.1

Adenosylmethionine--8-amino-7-oxononanoate transaminase: (8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)

Xcc-8004.1559.1

Dethiobiotin synthase: (7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)

Xcc-8004.4530.1

Biotin synthase: dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)

Xcc-8004.505.1

Biotin synthase: 4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)

Xcc-8004.505.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information