MetaCyc Pathway: cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) in Pseudomonas syringae pv. syringae B728a

Uroporphyrinogen-III C-methyltransferase: uroporphyrinogen-III + S-adenosyl-L-methionine→precorrin-1 + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.107)

Psyr_0060

Psyr_2098

Psyr_3174

Uroporphyrinogen-III C-methyltransferase: precorrin-1 + S-adenosyl-L-methionine→precorrin-2 + S-adenosyl-L-homocysteine (EC 2.1.1.107)

Psyr_0060

Psyr_2098

Psyr_3174

Precorrin-2 C(20)-methyltransferase: precorrin-2 + S-adenosyl-L-methionine→precorrin-3A + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.130)

Psyr_4415

Precorrin-3B synthase: precorrin-3A + NADH + dioxygen + H+→precorrin-3B + NAD+ + H2O (EC 1.14.13.83)

Psyr_4417

Precorrin-3B C(17)-methyltransferase: precorrin-3B + S-adenosyl-L-methionine→precorrin-4 + S-adenosyl-L-homocysteine + 2 H+ (EC 2.1.1.131)

Psyr_3019

Psyr_4414

Precorrin-4 C(11)-methyltransferase: precorrin-4 + S-adenosyl-L-methionine→precorrin-5 + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.133)

Psyr_3020

Precorrin-6A synthase (deacetylating): precorrin-5 + S-adenosyl-L-methionine + H2O→precorrin-6A + acetate + S-adenosyl-L-homocysteine + 2 H+ (EC 2.1.1.152)

Psyr_2442

Precorrin-6A reductase (in reverse): precorrin-6A + NADPH + H+→precorrin-6B + NADP+ (EC 1.3.1.54)

Psyr_4420

Precorrin-6Y C(5,15)-methyltransferase (decarboxylating): precorrin-6B + H+ + S-adenosyl-L-methionine→precorrin-7 + S-adenosyl-L-homocysteine + CO2 (EC 2.1.1.132)

Psyr_4418

Precorrin-6Y C(5,15)-methyltransferase (decarboxylating): precorrin-7 + S-adenosyl-L-methionine→precorrin-8x + S-adenosyl-L-homocysteine + 2 H+ (EC 2.1.1.132)

Psyr_4418

precorrin-8x + H+→hydrogenobyrinate (EC 5.4.99.61)

Psyr_4416

Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing): hydrogenobyrinate + 2 ATP + 2 L-glutamine + 2 H2O→hydrogenobyrinate a,c-diamide + 2 ADP + 2 L-glutamate + 2 H+ + 2 phosphate (EC 6.3.5.9)

Psyr_3680

Cobaltochelatase: hydrogenobyrinate a,c-diamide + ATP + Co2+ + H2O→cob(II)yrinate a,c-diamide + ADP + 3 H+ + phosphate (EC 6.6.1.2)

Psyr_3015

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information