MetaCyc Pathway: cob(II)yrinate <i>a,c</i>-diamide biosynthesis II (late cobalt incorporation) in Sinorhizobium meliloti 1021

MetaCyc Pathway: cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) in Sinorhizobium meliloti 1021

Uroporphyrinogen-III C-methyltransferase:
uroporphyrinogen-III + S-adenosyl-L-methionine→precorrin-1 + S-adenosyl-L-homocysteine + H⁺ (EC 2.1.1.107)

Uroporphyrinogen-III C-methyltransferase:
precorrin-1 + S-adenosyl-L-methionine→precorrin-2 + S-adenosyl-L-homocysteine (EC 2.1.1.107)

Precorrin-2 C(20)-methyltransferase:
precorrin-2 + S-adenosyl-L-methionine→precorrin-3A + S-adenosyl-L-homocysteine + H⁺ (EC 2.1.1.130)

Precorrin-3B synthase:
precorrin-3A + NADH + dioxygen + H⁺→precorrin-3B + NAD⁺ + H₂O (EC 1.14.13.83)

Precorrin-3B C(17)-methyltransferase:
precorrin-3B + S-adenosyl-L-methionine→precorrin-4 + S-adenosyl-L-homocysteine + 2 H⁺ (EC 2.1.1.131)

Precorrin-4 C(11)-methyltransferase:
precorrin-4 + S-adenosyl-L-methionine→precorrin-5 + S-adenosyl-L-homocysteine + H⁺ (EC 2.1.1.133)

Precorrin-6A synthase (deacetylating):
precorrin-5 + S-adenosyl-L-methionine + H₂O→precorrin-6A + acetate + S-adenosyl-L-homocysteine + 2 H⁺ (EC 2.1.1.152)

Precorrin-6A reductase (in reverse):
precorrin-6A + NADPH + H⁺→precorrin-6B + NADP⁺ (EC 1.3.1.54)

Precorrin-6Y C(5,15)-methyltransferase (decarboxylating):
precorrin-6B + H⁺ + S-adenosyl-L-methionine→precorrin-7 + S-adenosyl-L-homocysteine + CO₂ (EC 2.1.1.132)

Precorrin-6Y C(5,15)-methyltransferase (decarboxylating):
precorrin-7 + S-adenosyl-L-methionine→precorrin-8x + S-adenosyl-L-homocysteine + 2 H⁺ (EC 2.1.1.132)

precorrin-8x + H⁺→hydrogenobyrinate (EC 5.4.99.61)

Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing):
hydrogenobyrinate + 2 ATP + 2 L-glutamine + 2 H₂O→hydrogenobyrinate a,c-diamide + 2 ADP + 2 L-glutamate + 2 H⁺ + 2 phosphate (EC 6.3.5.9)

Cobaltochelatase:
hydrogenobyrinate a,c-diamide + ATP + Co²⁺ + H₂O→cob(II)yrinate a,c-diamide + ADP + 3 H⁺ + phosphate (EC 6.6.1.2)

Links:

Pathway details at MetaCyc
Fitness data for 17 genes
Select organism:
All MetaCyc pathways for Sinorhizobium meliloti 1021

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information