MetaCyc Pathway: peptidoglycan biosynthesis I (<I>meso</I>-diaminopimelate containing) in Sinorhizobium meliloti 1021

MetaCyc Pathway: peptidoglycan biosynthesis I (meso-diaminopimelate containing) in Sinorhizobium meliloti 1021

D-alanine--D-alanine ligase:
2 D-alanine + ATP→D-alanyl-D-alanine + ADP + H⁺ + phosphate (EC 6.3.2.4)

Glutamate racemase:
L-glutamate→D-glutamate (EC 5.1.1.3)

UDP-N-acetylglucosamine 1-carboxyvinyltransferase:
UDP-N-acetyl-α-D-glucosamine + phosphoenolpyruvate→UDP-N-acetyl-α-D-glucosamine-enolpyruvate + phosphate (EC 2.5.1.7)

UDP-N-acetyl-α-D-glucosamine-enolpyruvate + NADPH + H⁺→UDP-N-acetyl-α-D-muramate + NADP⁺ (EC 1.3.1.98)

UDP-N-acetylmuramate--L-alanine ligase:
UDP-N-acetyl-α-D-muramate + L-alanine + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanine + ADP + H⁺ + phosphate (EC 6.3.2.8)

UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase:
UDP-N-acetyl-α-D-muramoyl-L-alanine + D-glutamate + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + ADP + H⁺ + phosphate (EC 6.3.2.9)

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase:
UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + meso-diaminopimelate + ATP→UDP-MurNAc-L-Ala-γ-D-Glu-meso-DAP + ADP + H⁺ + phosphate (EC 6.3.2.13)

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase:
D-alanyl-D-alanine + UDP-MurNAc-L-Ala-γ-D-Glu-meso-DAP + ATP→UDP-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala + ADP + H⁺ + phosphate (EC 6.3.2.10)

Phospho-N-acetylmuramoyl-pentapeptide-transferase:
UDP-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala + di-trans,octa-cis-undecaprenyl phosphate→Und-PP-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala + UMP (EC 2.7.8.13)

acetylglucosaminyltransferase:
Und-PP-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala + UDP-N-acetyl-α-D-glucosamine→Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala + H⁺ + UDP (EC 2.4.1.227)

Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala_[in] + H⁺_[out]→Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala_[out] + H⁺_[in]

No genes

Peptidoglycan glycosyltransferase:
Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala + a nascent peptidoglycan with (L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine) pentapeptide→a nascent peptidoglycan with (L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine) pentapeptide + di-trans,octa-cis-undecaprenyl diphosphate (EC 2.4.1.129)

Links:

Pathway details at MetaCyc
Fitness data for 18 genes
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All MetaCyc pathways for Sinorhizobium meliloti 1021

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information