MetaCyc Pathway: L-arginine biosynthesis II (acetyl cycle) in Sinorhizobium meliloti 1021

MetaCyc Pathway: L-arginine biosynthesis II (acetyl cycle) in Sinorhizobium meliloti 1021

Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H⁺ + phosphate (EC 6.3.1.2)

Amino-acid N-acetyltransferase:
L-glutamate + acetyl-CoA→N-acetyl-L-glutamate + coenzyme A + H⁺ (EC 2.3.1.1)

Acetylglutamate kinase:
N-acetyl-L-glutamate + ATP→N-acetylglutamyl-phosphate + ADP (EC 2.7.2.8)

Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
L-glutamine + 2 ATP + hydrogen carbonate + H₂O→L-glutamate + carbamoyl phosphate + 2 ADP + 2 H⁺ + phosphate (EC 6.3.5.5)

N-acetyl-gamma-glutamyl-phosphate reductase (in reverse):
N-acetylglutamyl-phosphate + NADPH + H⁺→N-acetyl-L-glutamate 5-semialdehyde + NADP⁺ + phosphate (EC 1.2.1.38)

Ornithine carbamoyltransferase:
carbamoyl phosphate + L-ornithine→L-citrulline + H⁺ + phosphate (EC 2.1.3.3)

Acetylornithine transaminase (in reverse):
N-acetyl-L-glutamate 5-semialdehyde + L-glutamate→N-acetyl-L-ornithine + 2-oxoglutarate (EC 2.6.1.11)

Argininosuccinate synthase:
L-citrulline + ATP + L-aspartate→L-arginino-succinate + AMP + diphosphate + H⁺ (EC 6.3.4.5)

Argininosuccinate lyase:
L-arginino-succinate→L-arginine + fumarate (EC 4.3.2.1)

Glutamate N-acetyltransferase:
L-glutamate + N-acetyl-L-ornithine→N-acetyl-L-glutamate + L-ornithine (EC 2.3.1.35)

Links:

Pathway details at MetaCyc
Fitness data for 22 genes
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All MetaCyc pathways for Sinorhizobium meliloti 1021

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information