MetaCyc Pathway: biotin biosynthesis I in Shewanella amazonensis SB2B

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a malonyl-[acp] + S-adenosyl-L-methionine→a malonyl-[acp] methyl ester + S-adenosyl-L-homocysteine (EC 2.1.1.197)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a malonyl-[acp] + a malonyl-[acp] methyl ester + H+→a 3-oxoglutaryl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Sama_1978
Sama_2154
Sama_3391
Sama_3394
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxoglutaryl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxyglutaryl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
Sama_1381 (fabG)
Sama_1980
Sama_2250
Sama_3393 (fabG)
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyglutaryl-[acp] methyl ester→a (2E)-enoylglutaryl-[acp] methyl ester + H2O (EC 4.2.1.59)
Sama_1150 (fabZ)
Sama_1605
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylglutaryl-[acp] methyl ester + NADH + H+→a glutaryl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
Sama_1112
Sama_2734
Beta-ketoacyl-acyl-carrier-protein synthase II:
a glutaryl-[acp] methyl ester + a malonyl-[acp] + H+→a 3-oxopimeloyl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Sama_1978
Sama_2154
Sama_3391
Sama_3394
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxopimeloyl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxypimeloyl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
Sama_1381 (fabG)
Sama_1980
Sama_2250
Sama_3393 (fabG)
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxypimeloyl-[acp] methyl ester→a (2E)-enoylpimeloyl-[acp] methyl ester + H2O (EC 4.2.1.59)
Sama_1150 (fabZ)
Sama_1605
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylpimeloyl-[acp] methyl ester + NADH + H+→a pimeloyl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
Sama_1112
Sama_2734
a pimeloyl-[acp] methyl ester + H2O→a pimeloyl-[acp] + methanol + H+ (EC 3.1.1.85)
Sama_3505
8-amino-7-oxononanoate synthase:
a pimeloyl-[acp] + L-alanine + H+→(8S)-8-amino-7-oxononanoate + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.47)
Sama_1439
Adenosylmethionine--8-amino-7-oxononanoate transaminase:
(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)
Sama_1437
Dethiobiotin synthase:
(7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)
Sama_1441 (bioD)
Biotin synthase:
dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)
Sama_1438
Biotin synthase:
4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)
Sama_1438

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information