MetaCyc Pathway: tylosin biosynthesis in Rhodospirillum rubrum S1H

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(2S)-ethylmalonyl-CoA + 5 (S)-methylmalonyl-CoA + 2 malonyl-CoA + 7 NADPH + 15 H+→tylactone + 8 CO2 + 8 coenzyme A + 7 NADP+ + 3 H2O
No genes
tylactone + dTDP-α-D-mycaminose→5-O-β-mycaminosyltylactone + H+ + dTDP (EC 2.4.1.316)
No genes
5-O-β-mycaminosyltylactone + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→20-hydroxy-5-O-mycaminosyltylactone + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O
No genes
20-hydroxy-5-O-mycaminosyltylactone + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→20-oxo-5-O-β-mycaminosyltylactone + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
No genes
20-oxo-5-O-β-mycaminosyltylactone + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→5-O-β-mycaminosyltylonolide + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.34)
No genes
5-O-β-mycaminosyltylonolide + dTDP-6-deoxy-α-D-allose→demethyllactenocin + H+ + dTDP (EC 2.4.1.317)
No genes
demethyllactenocin + dTDP-β-L-mycarose→demethylmacrocin + H+ + dTDP (EC 2.4.1.318)
No genes
Demethylmacrocin O-methyltransferase:
demethylmacrocin + S-adenosyl-L-methionine→macrocin + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.102)
No genes
Macrocin O-methyltransferase:
macrocin + S-adenosyl-L-methionine→tylosin + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.101)
Rru_A2351

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information