MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Rhodopseudomonas palustris CGA009

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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
TX73_000555
TX73_008310
TX73_010315
TX73_010465
TX73_010470
TX73_012030
TX73_014300
TX73_019485
TX73_020305
TX73_021005
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
TX73_010485
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
TX73_007515
TX73_011115
TX73_012105
TX73_017970
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
TX73_001945
TX73_003400
TX73_003490
TX73_005430
TX73_006190
TX73_010080
TX73_010400
TX73_015865
TX73_018940
TX73_020325
TX73_025060

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information