MetaCyc Pathway: palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) in Shewanella loihica PV-4

Add experiment(s):


Beta-ketoacyl-acyl-carrier-protein synthase II:
a (5Z)-dodec-5-enoyl-[acp] + a malonyl-[acp] + H+→a (7Z)-3-oxotetradec-7-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
Shew_1605
Shew_2410
Shew_3523
Shew_3526
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (7Z)-3-oxotetradec-7-enoyl-[acp] + NADPH + H+→a (3R,7Z)-3-hydroxytetradec-7-enoyl-[acp] + NADP+
(EC 1.1.1.100)
Shew_1322
Shew_1326
Shew_1407
Shew_1603
Shew_1673 (fabG)
Shew_3525 (fabG)
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,7Z)-3-hydroxytetradec-7-enoyl-[acp]→a (2E,7Z)-tetradeca-2,7-dienoyl-[acp] + H2O
(EC 4.2.1.59)
Shew_1817
Shew_2624
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,7Z)-tetradeca-2,7-dienoyl-[acp] + NADH + H+→a (7Z)-tetradec-7-enoyl-[acp] + NAD+
(EC 1.3.1.9)
Shew_1878
Shew_2668
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (7Z)-tetradec-7-enoyl-[acp] + a malonyl-[acp] + H+→a (9Z)-3-oxohexadec-9-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
Shew_1605
Shew_2410
Shew_3523
Shew_3526
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (9Z)-3-oxohexadec-9-enoyl-[acp] + NADPH + H+→a (3R,9Z)-3-hydroxyhexadec-9-enoyl-[acp] + NADP+
(EC 1.1.1.100)
Shew_1322
Shew_1326
Shew_1407
Shew_1603
Shew_1673 (fabG)
Shew_3525 (fabG)
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,9Z)-3-hydroxyhexadec-9-enoyl-[acp]→a (2E,9Z)-hexadeca-2,9-dienoyl-[acp] + H2O
(EC 4.2.1.59)
Shew_1817
Shew_2624
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,9Z)-hexadeca-2,9-dienoyl-[acp] + NADH + H+→a palmitoleoyl-[acp] + NAD+
(EC 1.3.1.9)
Shew_1878
Shew_2668
Oleoyl-[acyl-carrier-protein] hydrolase:
a palmitoleoyl-[acp] + H2O→palmitoleate + a soluble [acyl-carrier protein] + H+
(EC 3.1.2.14)
Shew_2403

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information