MetaCyc Pathway: cholesterol biosynthesis (plants, early side-chain reductase) in Mycobacterium tuberculosis H37Rv

Add experiment(s):


Delta(24)-sterol reductase (in reverse):
cycloartenol + NADPH + H+→cycloartanol + NADP+ (EC 1.3.1.72)
No genes
cycloartanol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→(3β,9β)-4α-demethyl-4α-carboxy-9,19-cyclolanost-3-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.9)
No genes
(3β,9β)-4α-demethyl-4α-carboxy-9,19-cyclolanost-3-ol + NAD+→31-norcycloartanone + CO2 + NADH (EC 1.1.1.418)
No genes
3-keto-steroid reductase (in reverse):
31-norcycloartanone + NADPH + H+→31-norcycloartanol + NADP+ (EC 1.1.1.270)
No genes
Cycloeucalenol cycloisomerase:
31-norcycloartanol→4α,14α-dimethyl-cholesta-8-enol (EC 5.5.1.9)
No genes
4α,14α-dimethyl-cholesta-8-enol + 3 dioxygen + 3 a reduced [NADPH-hemoprotein reductase]→4α-methyl-cholesta-8,14-dienol + formate + 3 an oxidized [NADPH-hemoprotein reductase] + H+ + 4 H2O (EC 1.14.14.154)
Rv0764c
Delta(14)-sterol reductase (in reverse):
4α-methyl-cholesta-8,14-dienol + NADPH + H+→4α-methyl-5α-cholesta-8-en-3-ol + NADP+ (EC 1.3.1.70)
No genes
Cholestenol Delta-isomerase (in reverse):
4α-methyl-5α-cholesta-8-en-3-ol→4α-methyl-5α-cholest-7-en-3β-ol (EC 5.3.3.5)
No genes
4α-methyl-5α-cholest-7-en-3β-ol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→4α-carboxy-5α-cholest-7-en-3β-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.M9)
No genes
4α-carboxy-5α-cholest-7-en-3β-ol + NAD+→lathosterone + CO2 + NADH (EC 1.1.1.418)
No genes
3-keto-steroid reductase (in reverse):
lathosterone + NADPH + H+→lathosterol + NADP+ (EC 1.1.1.270)
No genes
lathosterol + 2 a ferrocytochrome b5 + dioxygen + 2 H+→7-dehydrocholesterol + 2 a ferricytochrome b5 + 2 H2O (EC 1.14.19.20)
No genes
7-dehydrocholesterol reductase (in reverse):
7-dehydrocholesterol + NADPH + H+→cholesterol + NADP+ (EC 1.3.1.21)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information