MetaCyc Pathway: tetracycline and oxytetracycline biosynthesis in Mycobacterium tuberculosis H37Rv

Add experiment(s):


6-methylpretetramide + NADPH + dioxygen + H+→4-hydroxy-6-methylpretetramide + NADP+ + H2O (EC 1.14.13.232)
No genes
4-hydroxy-6-methylpretetramide + NADPH + dioxygen + H+→4-dedimethylamino-4-oxoanhydrotetracycline + NADP+ + H2O (EC 1.14.13.233)
No genes
4-dedimethylamino-4-oxoanhydrotetracycline + an aminated amino group donor→4-amino-4-de(dimethylamino)anhydrotetracycline + a deaminated amino group donor (EC 2.6.1.M2)
Rv1178
4-amino-4-de(dimethylamino)anhydrotetracycline + S-adenosyl-L-methionine→4-methylamino-4-de(dimethylamino)anhydrotetracycline + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.335)
No genes
4-methylamino-4-de(dimethylamino)anhydrotetracycline + S-adenosyl-L-methionine→anhydrotetracycline + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.335)
No genes
Anhydrotetracycline monooxygenase:
anhydrotetracycline + NADPH + dioxygen + H+→5a,11a-dehydrotetracycline + NADP+ + H2O (EC 1.14.13.38)
No genes
5a,11a-dehydrotetracycline + NADPH + dioxygen + H+→5a,11a-dehydrooxytetracycline + NADP+ + H2O (EC 1.14.13.234)
No genes
5a,11a-dehydrotetracycline + a reduced factor 420→tetracycline + an oxidized factor 420 (EC 1.3.98.4)
No genes
5a,11a-dehydrooxytetracycline + a reduced factor 420→oxytetracycline + an oxidized factor 420 (EC 1.3.98.4)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information