MetaCyc Pathway: pyruvate fermentation to hexanol (engineered) in Marinobacter adhaerens HP15

MetaCyc Pathway: pyruvate fermentation to hexanol (engineered) in Marinobacter adhaerens HP15

Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO₂ + H⁺ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)

Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H⁺→(S)-3-hydroxybutanoyl-CoA + NAD⁺ (EC 1.1.1.35)

(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H₂O (EC 4.2.1.150)

Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H⁺→butanoyl-CoA + NAD⁺ (EC 1.3.1.44)

Acetyl-CoA C-acyltransferase:
acetyl-CoA + butanoyl-CoA→3-oxohexanoyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

3-hydroxyacyl-CoA dehydrogenase (in reverse):
3-oxohexanoyl-CoA + NADH + H⁺→(S)-3-hydroxyhexanoyl-CoA + NAD⁺ (EC 1.1.1.35)

Long-chain-enoyl-CoA hydratase:
(S)-3-hydroxyhexanoyl-CoA→(2E)-hex-2-enoyl-CoA + H₂O (EC 4.2.1.74)

Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
(2E)-hex-2-enoyl-CoA + NADH + H⁺→hexanoyl-CoA + NAD⁺ (EC 1.3.1.44)

No genes

hexanoyl-CoA + NAD(P)H + H⁺→1-hexanal + coenzyme A + NAD(P)⁺

No genes

1-hexanal + NADH + H⁺→hexan-1-ol + NAD⁺

No genes

Links:

Pathway details at MetaCyc
Fitness data for 36 genes
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All MetaCyc pathways for Marinobacter adhaerens HP15

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information