MetaCyc Pathway: oleate biosynthesis IV (anaerobic) in Pantoea sp. MT58

Add experiment(s):


3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
IAI47_12660
IAI47_15475
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dodec-2-enoyl-[acp]→a (3Z)-dodec-3-enoyl-[acp]
(EC 5.3.3.14)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dodec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxo-tetradec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
IAI47_06180
IAI47_12060
IAI47_12310
IAI47_12325
IAI47_21695
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxo-tetradec-5-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,5Z)-3-hydroxy-tetradec-5-enoyl-[acp][cytosol] + NADP+[cytosol]
(EC 1.1.1.100)
IAI47_07850
IAI47_11125
IAI47_12070
IAI47_12315
IAI47_15760
IAI47_16985
IAI47_19145
IAI47_19240
IAI47_20865
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxy-tetradec-5-enoyl-[acp][cytosol]→a (2E,5Z)-tetradeca-2,5-dienoyl-[acp][cytosol] + H2O[cytosol]
(EC 4.2.1.59)
IAI47_12660
IAI47_15475
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-tetradeca-2,5-dienoyl-[acp][cytosol] + NADH[cytosol] + H+[cytosol]→a (5Z)-tetradec-5-enoyl-[acp][cytosol] + NAD+[cytosol]
(EC 1.3.1.9)
IAI47_09355
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (5Z)-tetradec-5-enoyl-[acp] + a malonyl-[acp] + H+→a (7Z)-3-oxo-hexadec-7-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
IAI47_06180
IAI47_12060
IAI47_12310
IAI47_12325
IAI47_21695
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (7Z)-3-oxo-hexadec-7-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,7Z)-3-hydroxy-hexadec-7-enoyl-[acp][cytosol] + NADP+[cytosol]
(EC 1.1.1.100)
IAI47_07850
IAI47_11125
IAI47_12070
IAI47_12315
IAI47_15760
IAI47_16985
IAI47_19145
IAI47_19240
IAI47_20865
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,7Z)-3-hydroxy-hexadec-7-enoyl-[acp][cytosol]→a (2E,7Z)-hexadeca-2,7-dienoyl-[acp][cytosol] + H2O[cytosol]
(EC 4.2.1.59)
IAI47_12660
IAI47_15475
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,7Z)-hexadeca-2,7-dienoyl-[acp][cytosol] + NADH[cytosol] + H+[cytosol]→a (7Z)-hexadec-7-enoyl-[acp][cytosol] + NAD+[cytosol]
(EC 1.3.1.9)
IAI47_09355
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (7Z)-hexadec-7-enoyl-[acp] + a malonyl-[acp] + H+→a (9Z)-3-oxo-octadec-9-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
IAI47_06180
IAI47_12060
IAI47_12310
IAI47_12325
IAI47_21695
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (9Z)-3-oxo-octadec-9-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,9Z)-3-hydroxy-octadec-9-enoyl-[acp][cytosol] + NADP+[cytosol]
(EC 1.1.1.100)
IAI47_07850
IAI47_11125
IAI47_12070
IAI47_12315
IAI47_15760
IAI47_16985
IAI47_19145
IAI47_19240
IAI47_20865
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,9Z)-3-hydroxy-octadec-9-enoyl-[acp][cytosol]→a (2E,9Z)-octadeca-2,9-dienoyl-[acp][cytosol] + H2O[cytosol]
(EC 4.2.1.59)
IAI47_12660
IAI47_15475
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,9Z)-octadeca-2,9-dienoyl-[acp] + NADH + H+→an oleoyl-[acp] + NAD+
(EC 1.3.1.9)
IAI47_09355

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information