MetaCyc Pathway: superpathway of L-threonine metabolism in Klebsiella michiganensis M5al

MetaCyc Pathway: superpathway of L-threonine metabolism in Klebsiella michiganensis M5al

Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H₂O (EC 4.3.1.19)

L-threonine + ATP→L-threonine 3-O-phosphate + ADP + H⁺ (EC 2.7.1.177)

L-threonine 3-dehydrogenase:
L-threonine + NAD⁺→L-2-amino-3-oxobutanoate + NADH + H⁺ (EC 1.1.1.103)

L-threonine→acetaldehyde + glycine (EC 4.1.2.48; 4.1.2.5)

Threonine-phosphate decarboxylase:
L-threonine 3-O-phosphate + H⁺→(R)-1-amino-2-propanol O-2-phosphate + CO₂ (EC 4.1.1.81)

Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD⁺→acetyl-CoA + NADH + H⁺ (EC 1.2.1.10)

Glycine C-acetyltransferase (in reverse):
L-2-amino-3-oxobutanoate + coenzyme A→glycine + acetyl-CoA (EC 2.3.1.29)

1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

L-threonine 3-dehydrogenase:
L-2-amino-3-oxobutanoate + H⁺→aminoacetone + CO₂ (spontaneous) (EC 1.1.1.103; 1.1.1.381)

Primary-amine oxidase:
aminoacetone + dioxygen + H₂O→methylglyoxal + ammonium + hydrogen peroxide (EC 1.4.3.21)

2-iminobutanoate + H₂O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

Acetolactate synthase:
2-oxobutanoate + H⁺ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO₂ (EC 2.2.1.6)

Formate C-acetyltransferase (in reverse):
2-oxobutanoate + coenzyme A→propanoyl-CoA + formate (EC 2.3.1.54)

Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H⁺→(R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ (EC 1.1.1.86)

propanoyl-CoA + phosphate→propanoyl phosphate + coenzyme A (EC 2.3.1.222)

Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H₂O (EC 4.2.1.9)

Acetate kinase (in reverse):
propanoyl phosphate + ADP→propanoate + ATP (EC 2.7.2.1; 2.7.2.14; 2.7.2.15; 2.7.2.7)

Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

Links:

Pathway details at MetaCyc
Fitness data for 38 genes
Select organism:
All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information